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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat CD56/NCAM-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat CD56/NCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ncam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CD56/NCAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1562)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L20-T712
625 pg/ml - 40,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1562 is reactive to Ncam1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1562 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1562-CAP||Anti-Rat Ncam1 Pre-coated 96-well strip microplate||1|
|EK1562-ST||Rat Ncam1 Standard||2 vials, 40 ng/tube|
|EK1562-DA||Rat Ncam1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat NCAM1/CD56 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect NCAM-1/CD56, Dilution ratio of 1:50, concentration in serum is 49.7-291ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CD56/NCAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of NCAM-1/CD56 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ncam1 (Source: Uniprot.Org, NCBI)
Neural cell adhesion molecule 1
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM Ncam1|Cd56, N-CAM, N-CAM-1, NCAM-1, NCAM-C, NCAMC, Ncam|neural cell adhesion molecule 1|neural cell adhesion molecule 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ncam1, check out the Ncam1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ncam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1562 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zheng Y,Huang C,Yang X,Zhang Z.Altered expression of glycoprotein non‑metastatic melanoma protein B in the distal sciatic nerve following injury.Int J Mol Med.2020 Jun;45(6):1909-1917.doi:10.3892/ijmm.2020.4559.Epub 2020 Mar 31.PMID:32236569;PMCID:PMC7169
EK1562 usage in article: APP:ELISA, SAMPLE:SCS, DILUTION:NA
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