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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat Neurotrophin-3 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntf3 in cell culture supernatants and serum. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Neurotrophin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y139-T257
15.6 pg/ml - 1,000 pg/ml
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.
EK0474 is reactive to Ntf3 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK0474 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of NT-3
Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.
|EK0474-CAP||Anti-Rat Ntf3 Pre-coated 96-well strip microplate||1|
|EK0474-ST||Rat Ntf3 Standard||2 vials, 10 ng/tube|
|EK0474-DA||Rat Ntf3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat Neurotrophin-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect NT-3, Dilution ratio of 1:1.
Some articles we found to cite concentrations of NT-3 in samples: 23074106 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Neurotrophin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of NT-3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ntf3 (Source: Uniprot.Org, NCBI)
HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3 Ntf3|neurotrophin 3|neurotrophin-3|HDNF|NGF-2|NT-3|nerve growth factor 2|neurotrophic factor|neurotrophin-3 (HDNF/NT-3)*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ntf3, check out the Ntf3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0474 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats.
Electroacupuncture Promotes the Differentiation of Transplanted Bone Marrow Mesenchymal Stem Cells Overexpressing TrkC into Neuron-Like Cells in Transected Spinal Cord of Rats:
Triptolide improves neurobehavioral functions, inflammation, and oxidative stress in rats under deep hypothermic circulatory arrest
Swimming Training Reduces Neuroma Pain by Regulating Neurotrophins
Governor Vessel Electro-Acupuncture Promotes the Intrinsic Growth Ability of Spinal Neurons through Activating Calcitonin Gene-Related Peptide/α-Calcium/Calmodulin-Dependent Protein Kinase/Neurotrophin-3 Pathway after Spinal Cord Injury
Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats
Combination of adenoviral vector-mediated neurotrophin-3 gene transfer and retinoic acid promotes adult bone marrow cells to differentiate into neuronal phenotypes
Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments
An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury
Low-intensity pulsed ultrasound regulates proliferation and differentiation of neural stem cells through notch signaling pathway
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