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Rat Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntf3 in cell culture supernatants and serum.. Sensitivity: 2pg/ml. Cited in 5 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants and serum.|
|Sample Volume:||100μl per well|
|Assay Range:||15.6 pg/ml - 1,000 pg/ml|
|Product Name||Rat Neurotrophin-3 ELISA Kit PicoKine™
See more NT-3 products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Rat Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntf3 in cell culture supernatants and serum.. Sensitivity: 2pg/ml.|
|Cite This Product||Rat Neurotrophin-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants and serum.|
|Assay Range||15.6 pg/ml - 1,000 pg/ml|
|Immunogen/Standard||Expression system for standard: sf21; Immunogen sequence: Y139-T257|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||81737|
|Species Of This Entry||Rat|
|Alternative Names||NT-3|HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0474-CAP||Anti-Rat Ntf3 Pre-coated 96-well strip microplate||1|
|EK0474-ST||Rat Ntf3 Standard||2 vials, 10 ng/tube|
|EK0474-DA||Rat Ntf3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0474 Rat Neurotrophin-3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat Neurotrophin-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat Neurotrophin-3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Ge Li,Bao Zhang,Jia-hui Sun,Li-yang Shi,Meng-yao Huang,Li-jun Huang,Zi-jing Lin,Qiong-yu Lin,Bi-qin Lai,Yuan-huan Ma,Bin Jiang,Ying Ding,Hong-bo Zhang,Miao-xin Li,Ping Zhu,Ya-qiong Wang,Xiang Zeng,Yuan-shan Zeng,An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem cell-derived neural network tissue with efficacy in repairing spinal cord injury,Bioactive Materials,Volume 6,Issue 11,2021,Pages 3766-3781,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.03.036.
EK0474 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
|Xu H,Yang Y,Deng QW,Zhang BB,Ruan JW,Jin H,Wang JH,Ren J,Jiang B,Sun JH,Zeng Y,Ding Y. Governor Vessel electro-acupuncture promotes the intrinsic growth ability of spinal neurons through activating CGRP/αCaMKII/NT-3 pathway after spinal cord injury. J Neu
EK0474 usage in article: APP:ELISA, SAMPLE:SPINAL CORD TISSUES EXTRACTS, DILUTION:NA
|PubMed ID 33121345|
|Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats||PubMed ID 25779025|
|Zhao Z, Wang Y, Peng J, Ren Z, Zhang L, Guo Q, Xu W, Lu S. Cell Transplant. 2014 Jan;23(1):97-110. Doi: 10.3727/096368912X658845. Epub 2012 Nov 1. Improvement In Nerve Regeneration Through A Decellularized Nerve Graft By Supplementation With Bone ...||PubMed ID 23128095|
|Wang Y, Li J, Kong P, Zhao S, Yang H, Chen C, Yan J. Spine (Phila Pa 1976). 2015 Jan 15;40(2):95-101. Doi: 10.1097/Brs.0000000000000694. Enhanced Expression Of Neurotrophic Factors In The Injured Spinal Cord Through Vaccination With Myelin Basic P...||PubMed ID 25569526|
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Comprehensive info on Ntf3, beautifully presented.Open Infographic
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