Overview
| Product Name |
Rat Neurotrophin-3 ELISA Kit PicoKine™
See more information about NT-3, and browse related primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0474 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Rat Neurotrophin 3/NTF3 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Neurotrophin 3/NTF3 tissue specificity: Brain and peripheral tissues. |
| Cite This Product |
Rat Neurotrophin-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates and serum. |
| Sensitivity |
<2pg/ml |
| Assay Range |
15.6pg/ml-1000pg/ml |
| Immunogen/Standard |
Expression system for standard: sf21; Immunogen sequence: Y139-T257 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Ntf3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P18280 |
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| NCBI Gene Id | 81737 |
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| Species Of This Entry | Rat |
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| Gene Name | Ntf3 |
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| Protein Name | Neurotrophin-3 |
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| Superfamily | NGF-beta family |
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| Alternative Names | NT-3|HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3 |
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| Molecular Weight | 29645 |
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| Post Tranlational Modifications | Phosphorylation; Cleavage; Methylation |
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| Gene Location | 4q42, on Chromasome 4, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ntf3 (Source: Uniprot.org, NCBI)
| Protein Function | Seems to promote the survival of visceral and proprioceptive sensory neurons. |
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| Tissue Specificity | Brain and peripheral tissues. |
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| Research Areas | Apoptosis; Cancer |
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| Related Diseases | Nervousness; Attention Deficit Hyperactivity Disorder; Dysequilibrium Syndrome; Malnutrition; Schizophrenia; Cholangiocarcinoma; Neoplasm Metastasis; Malignant Neoplasm Of Breast; Adolescent Idiopathic Scoliosis; Glioma; Glaucoma, Open-angle; Neuronal Migration Disorders; Infertility; Malignant Neoplasms; Neoplasms; Scoliosis, Unspecified; 5,10-methylenetetrahydrofolate Reductase Deficiency |
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| Related Pathways | Pathogenesis; Transport; Cell Migration; Sex Determination; Cell Division; Cell Differentiation; Innervation; Cell Adhesion; Sertoli Cell Differentiation; Fertilization; Brain Development; Eating Behavior; Leydig Cell Differentiation; Ovule Development; Spermatogenesis; Reflex; Autocrine Signaling; Translation; Gliogenesis; Gonad Development |
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| Background | Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6. |
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| Scientific References | PMID: 2164684 by Ernfors P., et al. Molecular cloning and neurotrophic activities of a protein with structural similarities to nerve growth factor: developmental and topographical expression in the brain.
PMID: 2321006 by Maisonpierre P.C., et al. Neurotrophin-3: a neurotrophic factor related to NGF and BDNF. |
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Kit Components And Assay QC Data Details Of Rat Neurotrophin-3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0474-CAP | 96-well plate precoated with anti-Rat NT-3 antibody | 1 |
| EK0474-ST | lyophilized recombinant Rat NT-3 standard | 10ng/tube |
| EK0474-DA | biotinylated anti-Rat NT-3 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| - | Wash Buffer | Powder pack for 1000ml |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0474 Rat Neurotrophin-3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.003 | 0.056 | 0.097 | 0.233 | 0.388 | 0.791 | 1.486 | 2.203 |
We measured random samples of Rat Neurotrophin-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 41 | 173 | 464 | 41 | 177 | 504 |
| Standard deviation | 2.25 | 12.45 | 23.66 | 2.99 | 15.53 | 27.72 |
| CV(%) | 5.5% | 7.2% | 5.5% | 7.3% | 9% | 5.5% |
We measured the performance consistency of Rat Neurotrophin-3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 41 | 38 | 39 | 39 | 39 | 1.08 | 2.7% |
| Sample 2 | 173 | 159 | 176 | 168 | 169 | 6.44 | 3.8% |
| Sample 3 | 464 | 416 | 419 | 479 | 444 | 27.53 | 6.2% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 3
| Wang Y, Li J, Kong P, Zhao S, Yang H, Chen C, Yan J. Spine (Phila Pa 1976). 2015 Jan 15;40(2):95-101. Doi: 10.1097/Brs.0000000000000694. Enhanced Expression Of Neurotrophic Factors In The Injured Spinal Cord Through Vaccination With Myelin Basic P... |
PubMed ID 25569526 |
| Zhao Z, Wang Y, Peng J, Ren Z, Zhang L, Guo Q, Xu W, Lu S. Cell Transplant. 2014 Jan;23(1):97-110. Doi: 10.3727/096368912X658845. Epub 2012 Nov 1. Improvement In Nerve Regeneration Through A Decellularized Nerve Graft By Supplementation With Bone ... |
PubMed ID 23128095 |
| Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats |
PubMed ID 25779025 |
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Questions and answers from customers related to EK0474 Rat Neurotrophin-3 ELISA Kit PicoKine™
1 Related Questions
Question
Are the baculovirus expressing systems used for manufacturing the kits?
Keyword: expression system
Verified Customer
Asked: 2019-04-11
Answer
sf21 was the expression system for both the standard and recombinant protein immunogen for NT3 ELISA kit.
Boster Scientific Support
Answered: 2019-04-11
