|Size||96wells/kit, with removable strips.|
|Sample Type||cell culture supernates and serum.|
|Product Name||Rat Neurotrophin-3 PicoKine™ ELISA Kit|
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Size||96wells/kit, with removable strips.|
|Description||Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Neurotrophin-3. 96wells/kit, with removable strips.|
|Cite This Product||Rat Neurotrophin-3 PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)|
|Sample Type||cell culture supernates and serum..|
|Immunogen||Expression system for standard: sf21; Immunogen sequence: Y139-T257|
|Cross Reactivity||There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine.|
|Antibody Clonalities||Capture Antibody | Detection Antibody:
polyclonal antibody from goat|polyclonal antibody from goat
|EK0474-CAP||96-well plate precoated with anti-Rat NTF3 antibody||1|
|EK0474-ST||lyophilized recombinant Rat NTF3 standard||10ng/tube|
|EK0474-DA||biotinylated anti-Rat NTF3 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Rat Neurotrophin-3 PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision||Inter-Assay Precision|
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Protein Target Info (Source: Uniprot.org)
|Tissue Specificity||Brain and peripheral tissues.|
|Alternative Names||Neurotrophin-3;NT-3;HDNF;Nerve growth factor 2;NGF-2;Neurotrophic factor;Ntf3;Ntf-3;|
|Molecular Weight||29645 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Seems to promote the survival of visceral and proprioceptive sensory neurons.|
|Research Areas||Growth And Development, Neurology Process, Neuroscience
*You can search these to find other products in these research areas.
|Background||Neurotrophin-3(NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3(Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest(NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.|
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1. Diluent the samples with the provided sample diluent buffer into 100ul.
2. Add 50ul of standard solution, when 50ul of sample will be added into a well.
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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