Overview
| Product Name |
Rat Neurotrophin-3 ELISA Kit PicoKine™
See more NT-3 products |
| Catalog# |
EK0474 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Rat Neurotrophin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntf3 in cell culture supernatants and serum.. Sensitivity: 2pg/ml. |
| Cite This Product |
Rat Neurotrophin-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0474)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants and serum. |
| Sensitivity |
<2 pg/ml |
| Assay Range |
15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: sf21; Immunogen sequence: Y139-T257 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is cross-reactivity with NT-4<2.5%, and no detectable cross-reactivity with any other cytokine. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Ntf3 (Source: Uniprot.org, NCBI)
| Uniprot Id | P18280 |
|---|
| NCBI Gene Id | 81737 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Ntf3 |
|---|
| Protein Name | Neurotrophin-3 |
|---|
| Superfamily | NGF-beta family |
|---|
| Alternative Names | NT-3|HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; neurotrophin 3; neurotrophin-3; NGF2; NGF-2; NT3; NT-3; NTF3 |
|---|
| Molecular Weight | 29645 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ntf3, check out the Ntf3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ntf3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Ntf3 infographic
Kit Components And Assay QC Data Details Of Rat Neurotrophin-3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0474-CAP |
Anti-Rat Ntf3 Pre-coated 96-well strip microplate | 1 |
| EK0474-ST | Rat Ntf3 Standard | 2 vials, 10 ng/tube |
| EK0474-DA | Rat Ntf3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0474 Rat Neurotrophin-3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.003 | 0.056 | 0.097 | 0.233 | 0.388 | 0.791 | 1.486 | 2.203 |
We measured random samples of Rat Neurotrophin-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 41 | 173 | 464 | 41 | 177 | 504 |
| Standard deviation | 2.25 | 12.45 | 23.66 | 2.99 | 15.53 | 27.72 |
| CV (%) | 5.5% | 7.2% | 5.5% | 7.3% | 9% | 5.5% |
We measured the performance consistency of Rat Neurotrophin-3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 41 | 38 | 39 | 39 | 39 | 1.08 | 2.7% |
| Sample 2 | 173 | 159 | 176 | 168 | 169 | 6.44 | 3.8% |
| Sample 3 | 464 | 416 | 419 | 479 | 444 | 27.53 | 6.2% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 4
Xu H,Yang Y,Deng QW,Zhang BB,Ruan JW,Jin H,Wang JH,Ren J,Jiang B,Sun JH,Zeng Y,Ding Y. Governor Vessel electro-acupuncture promotes the intrinsic growth ability of spinal neurons through activating CGRP/αCaMKII/NT-3 pathway after spinal cord injury. J Neu
Species: Rat
EK0474 usage in article: APP:ELISA, SAMPLE:SPINAL CORD TISSUES EXTRACTS, DILUTION:NA |
PubMed ID 33121345 |
| Combination of Electroacupuncture and Grafted Mesenchymal Stem Cells Overexpressing TrkC Improves Remyelination and Function in Demyelinated Spinal Cord of Rats |
PubMed ID 25779025 |
| Zhao Z, Wang Y, Peng J, Ren Z, Zhang L, Guo Q, Xu W, Lu S. Cell Transplant. 2014 Jan;23(1):97-110. Doi: 10.3727/096368912X658845. Epub 2012 Nov 1. Improvement In Nerve Regeneration Through A Decellularized Nerve Graft By Supplementation With Bone ... |
PubMed ID 23128095 |
| Wang Y, Li J, Kong P, Zhao S, Yang H, Chen C, Yan J. Spine (Phila Pa 1976). 2015 Jan 15;40(2):95-101. Doi: 10.1097/Brs.0000000000000694. Enhanced Expression Of Neurotrophic Factors In The Injured Spinal Cord Through Vaccination With Myelin Basic P... |
PubMed ID 25569526 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0474 Rat Neurotrophin-3 ELISA Kit PicoKine™
9 Related Questions
Question
Are the baculovirus expressing systems used for manufacturing the kits?
Keyword: expression system
Verified Customer
Asked: 2019-04-11
Answer
sf21 was the expression system for both the standard and recombinant protein immunogen for NT3 ELISA kit.
Boster Scientific Support
Answered: 2019-04-11
Question
Q: how to proceed with the analysis of ELISA data? I have obtained NT-3 level in serum.
B. Hall
Asked: 2020-12-03
Boster Scientific Support
Answered: 2020-12-03
Question
Q: if the enzyme conjugated NT-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the NT-3 antigen?
K. Martinez
Asked: 2020-11-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-05
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Singh
Asked: 2019-12-19
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-19
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Collins
Asked: 2018-11-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: What is the optimal O.D. value for NT-3 ELISA kit? I used your NT-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain NT-3 even though the O.D. values are not very high?
Verified Customer
Asked: 2017-10-13
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this NT-3 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of NT-3 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2017-10-13
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring NT-3 using the EK0474 Rat NT-3 Picokine® ELISA Kit?
B. Varma
Asked: 2017-08-03
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-08-03
Question
Q: how can I thaw whole blood sample for NT-3 ELISA after freezing?
J. Smith
Asked: 2017-04-21
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test NT-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-04-21
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of NT-3 in Rat serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
R. Krishna
Asked: 2014-07-07
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the NT-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-07-07
