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Rat NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum.. Sensitivity: 1pg/ml. Cited in 14 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants and serum.|
|Sample Volume:||100μl per well|
|Assay Range:||15.6 pg/ml - 1,000 pg/ml|
|Product Name||Rat NGF/NGF Beta ELISA Kit PicoKine™
See more beta-NGF products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Rat NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum.. Sensitivity: 1pg/ml.|
|Cite This Product||Rat NGF/NGF Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0471)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants and serum.|
|Assay Range||15.6 pg/ml - 1,000 pg/ml|
|Immunogen/Standard||Expression system for standard: NS0; Immunogen sequence: S122-G241|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||310738|
|Species Of This Entry||Rat|
|Protein Name||Beta-nerve growth factor|
|Alternative Names||beta-NGF|Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ngf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0471-CAP||Anti-Rat Ngf Pre-coated 96-well strip microplate||1|
|EK0471-ST||Rat Ngf Standard||2 vials, 10 ng/tube|
|EK0471-DA||Rat Ngf Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0471 Rat NGF/NGF Beta ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat NGF/NGF Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat NGF/NGF Beta ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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|Chen Y,Guo Y,Gharibani P,Chen J,Selaru FM,Chen JDZ.Transitional changes in gastrointestinal transit and rectal sensitivity from active to recovery of inflammation in a rodent model of colitis.Sci Rep.2021 Apr 15;11(1):8284.doi:10.1038/s41598-021-87814-7.PMID:33859347;PMCID:PMC8050040.
EK0471 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA
|PubMed ID 33859347|
|Shuo Liu,Caixia Fan,Yuanyuan Xie et al.Allogeneic Neural Stem Cell Transplant Promotes Functional Recovery of Locomotion After Complete Transected Spinal Cord Injury Predominantly by Secreting Neurotrophic Factors, 12 January 2021, PREPRINT (Version 1) av
EK0471 usage in article: APP:ELISA, SAMPLE:NSC, DILUTION:NA
|Zhu, Y.,Jin, Z.,Wang, J. et al.Ultrasound-guided platelet-rich plasma injection and multimodality ultrasound examination of peripheral nerve crush injury. npj Regen Med 5,21(2020).https://doi.org/10.1038/s41536-020-00101-3
Species: White Rabbit,Rat
EK0471 usage in article: APP:ELISA, SAMPLE:SCHWANN CELLS, DILUTION:NA
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|Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats||PubMed ID 26649780|
|Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials||PubMed ID 25484582|
|The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration:?In Vitro?Studies||PubMed ID 25133160|
|Sun X, Zhu Y, Yin HY, Guo ZY, Xu F, Xiao B, Jiang WL, Guo WM, Meng HY, Lu SB, Wang Y, Peng J. Stem Cell Res Ther. 2018 May 11;9(1):133. doi: 10.1186/s13287-018-0884-3. Differentiation of adipose-derived stem cells into Schwann cell-like cells thro...||PubMed ID 29751848|
|Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.||PubMed ID 26170829|
|Tan T, Xie J, Liu T, Chen X, Zheng X, Tong Z, Tian X. Exp Gerontol. 2013 Aug;48(8):786-94. Doi: 10.1016/J.Exger.2013.05.001. Epub 2013 May 9. Low-Frequency (1 Hz) Repetitive Transcranial Magnetic Stimulation (Rtms) Reverses A??(1-42)-Mediated Memo...||PubMed ID 23665072|
|Sisman Ar, Kiray M, Camsari Um, Evren M, Ates M, Baykara B, Aksu I, Guvendi G, Uysal N. Dis Markers. 2014;2014:108106. Doi: 10.1155/2014/108106. Epub 2014 Mar 20. Potential Novel Biomarkers For Diabetic Testicular Damage In Streptozotocin-Induced ...||PubMed ID 24771956|
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Comprehensive info on Ngf, beautifully presented.Open Infographic
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