Rat NGF/NGF Beta ELISA Kit PicoKine®

Product Name

Rat NGF/NGF Beta ELISA Kit PicoKine®

SKU/Catalog Number

EK0471

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat NGF/NGF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat NGF/NGF Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0471)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: S122-G241

Capture/Detection Antibodies

The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Standard Dilution Instructions

See datasheet of EK0471 for more details

Cross-reactivity

There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Reactive Species

EK0471 is reactive to Ngf in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0471 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Ngf

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

Kit Components

Catalog Number	Description	Quantity
EK0471-CAP	Anti-Rat Ngf Pre-coated 96-well strip microplate	1
EK0471-ST	Rat Ngf Standard	2 vials, 10 ng/tube
EK0471-DA	Rat Ngf Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.045	0.087	0.124	0.198	0.363	0.66	1.159	1.889

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Ngf, Dilution ratio of 1:1, concentration in serum is less than the lowest standard, 15.6 pg/ml..

Some articles we found to cite concentrations of Ngf in samples: 15615500 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat NGF/NGF Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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