Rat NGF/NGF Beta ELISA Kit PicoKine™

Rat NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml. Cited in 16 publication(s).

Product Info Summary

SKU: EK0471
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Rat NGF/NGF Beta ELISA Kit PicoKine™

See all beta-NGF products

SKU/Catalog Number

EK0471

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat NGF/NGF Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat NGF/NGF Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0471)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S122-G241

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Reactive Species

EK0471 is reactive to Ngf in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0471 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0471-CAP Anti-Rat Ngf Pre-coated 96-well strip microplate 1
EK0471-ST Rat Ngf Standard 2 vials, 10 ng/tube
EK0471-DA Rat Ngf Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0790.1330.1900.2960.5210.8761.4142.025

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat NGF/NGF Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3319148029201491
Standard deviation1.848.0232.641.6211.6535.84
CV (%)4.2%4.2%6.8%5.6%5.8%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of beta-NGF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133323335331.083.2%
Sample 21911721851921857.964.3%
Sample 348045144043645117.213.8%
*number of samples for each test n=16.

Gene/Protein Information For Ngf (Source: Uniprot.Org, NCBI)

Uniprot ID

P25427

Gene ID

310738

Gene Name

Ngf

Full Name

Beta-nerve growth factor

Weight

27.01kDa

Superfamily

NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 Ngf|Ngfb, beta-NGF|nerve growth factor|beta-nerve growth factor|nerve growth factor (beta polypeptide)|pro-nerve growth factor long

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ngf, check out the Ngf Infographic

Ngf infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ngf: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0471 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering
Species: Rat

Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces
Species: Rat

Chen Y,Guo Y,Gharibani P,Chen J,Selaru FM,Chen JDZ.Transitional changes in gastrointestinal transit and rectal sensitivity from active to recovery of inflammation in a rodent model of colitis.Sci Rep.2021 Apr 15;11(1):8284.doi:10.1038/s41598-021-87814-7.PMID:33859347;PMCID:PMC8050040.
Species: Rat
EK0471 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Shuo Liu,Caixia Fan,Yuanyuan Xie et al.Allogeneic Neural Stem Cell Transplant Promotes Functional Recovery of Locomotion After Complete Transected Spinal Cord Injury Predominantly by Secreting Neurotrophic Factors, 12 January 2021, PREPRINT (Version 1) av
Species: Rat
EK0471 usage in article: APP:ELISA, SAMPLE:NSC, DILUTION:NA

Zhu, Y.,Jin, Z.,Wang, J. et al.Ultrasound-guided platelet-rich plasma injection and multimodality ultrasound examination of peripheral nerve crush injury. npj Regen Med 5,21(2020).https://doi.org/10.1038/s41536-020-00101-3
Species: White Rabbit,Rat
EK0471 usage in article: APP:ELISA, SAMPLE:SCHWANN CELLS, DILUTION:NA

Electroactive biodegradable polyurethane significantly enhanced Schwann cells myelin gene expression and neurotrophin secretion for peripheral nerve tissue engineering

Effect of melatonin supplemented at the light or dark period on recovery of sciatic nerve injury in rats

Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron?astrocyte co-culture system through neurotrophy and neuroinflammation pathway

Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats

Effect of Zhuang Jing Decoction on Learning and Memory Ability in Aging Rats

Nanotechnology versus stem cell engineering: in vitro comparison of neurite inductive potentials

The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration:?In Vitro?Studies

Sun X, Zhu Y, Yin HY, Guo ZY, Xu F, Xiao B, Jiang WL, Guo WM, Meng HY, Lu SB, Wang Y, Peng J. Stem Cell Res Ther. 2018 May 11;9(1):133. doi: 10.1186/s13287-018-0884-3. Differentiation of adipose-derived stem cells into Schwann cell-like cells thro...

Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.

Tan T, Xie J, Liu T, Chen X, Zheng X, Tong Z, Tian X. Exp Gerontol. 2013 Aug;48(8):786-94. Doi: 10.1016/J.Exger.2013.05.001. Epub 2013 May 9. Low-Frequency (1 Hz) Repetitive Transcranial Magnetic Stimulation (Rtms) Reverses A??(1-42)-Mediated Memo...

Sisman Ar, Kiray M, Camsari Um, Evren M, Ates M, Baykara B, Aksu I, Guvendi G, Uysal N. Dis Markers. 2014;2014:108106. Doi: 10.1155/2014/108106. Epub 2014 Mar 20. Potential Novel Biomarkers For Diabetic Testicular Damage In Streptozotocin-Induced ...

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13 Customer Q&As for Rat NGF/NGF Beta ELISA Kit PicoKine™

Question

Is the standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) natural material or recombinant?

Verified customer

Asked: 2020-12-09

Answer

For the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471), the standard is recombinant Rat beta-NGF standard and expression system is NS0.

Boster Scientific Support

Answered: 2020-12-10

Question

If the standard for EK0471 is recombinant, does it use a virus vector in the recombination and/or expression process? What kind of virus vector was used?

Verified customer

Asked: 2020-12-09

Answer

The Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) didn't use any virus vector in the recombination and/or expression process.

Boster Scientific Support

Answered: 2020-12-10

Question

Does the standard for EK0471 include additive agent such as antiseptic agent or preserving agent? What is composition of additive agent?

Verified customer

Asked: 2020-12-09

Answer

The standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) includes 0.02mg sodium azide. It is 0.38% in the lyophilized state.

Boster Scientific Support

Answered: 2020-12-10

Question

What is the material name and concentration of Stop Solution for EK0471?

Verified customer

Asked: 2020-12-09

Answer

The stop solution for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) is 2% Sulphuric acid.

Boster Scientific Support

Answered: 2020-12-10

Question

Does EK0471 include hazardous substance such as azide, thimerosal, 2-ME, not less than 1% surfactant like CAS# 9002-93-1 or CAS# 9016-45-9, etc? What kind of substance is included in which component and how much?

Verified customer

Asked: 2020-12-09

Answer

The the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) contains sodium azide less than 1%.

Boster Scientific Support

Answered: 2020-12-10

Question

How was the standard for EK0471 purified?

Verified customer

Asked: 2020-12-08

Answer

The standard for the Rat NGF/NGF Beta ELISA Kit PicoKine™ (EK0471) was affinity purified.

Boster Scientific Support

Answered: 2020-12-09

Question

Q: which procedure should I follow in order to thaw whole blood sample for beta-NGF ELISA after freezing?

R. Baker

Verified customer

Asked: 2019-11-08

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test beta-NGF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-11-08

Question

Good day! I hope you are doing well. We received an inquiry about r.NGF/NGF (catalog# EK0471) from our customer today. Do you have a recommended dilution factor of serum sample? Or do you have any tested data for serum to share with the customer? Thank you very much for your help.

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

For EK0471, the recommended dilution factor of serum sample is 1:1.

Boster Scientific Support

Answered: 2019-09-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-12-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-10

Question

Q: can you recommend the dilution ratio of serum samples for detection of beta-NGF in Rat serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

W. Turner

Verified customer

Asked: 2018-12-02

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the beta-NGF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-12-02

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-05-19

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-05-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-05-28

Question

Q: Can beta-NGF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2014-07-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-07-01

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