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Product Info Summary
| SKU: | EK1209 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat SAP/PTX2/APCS ELISA Kit PicoKine®
View all Pentraxin 2/SAP ELISA kits
SKU/Catalog Number
EK1209
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SAP/PTX2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Apcs in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SAP/PTX2/APCS ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1209)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q21-S228
Sensitivity
<50 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1209 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1209 is reactive to APCS in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1209 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Pentraxin 2/SAP
Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1209-CAP | Anti-Rat APCS Pre-coated 96-well strip microplate | 1 |
| EK1209-ST | Rat APCS Standard | 2 vials, 100 ng/tube |
| EK1209-DA | Rat APCS Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.007 | 0.168 | 0.271 | 0.454 | 0.859 | 1.286 | 1.790 | 2.130 |
Data Example Images
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Rat SAP/PTX2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Pentraxin 2/SAP, Dilution ratio of 1:50000, concentration in serum and plasma is around 30 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SAP/PTX2/APCS ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 289 | 813 | 3769 | 294 | 1163 | 4012 |
| Standard deviation | 13.87 | 46.34 | 173.37 | 14.99 | 68.62 | 256.77 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Pentraxin 2/SAP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 273 | 296 | 296 | 294 | 289 | 9.70 | 3.3% |
| Sample 2 | 768 | 781 | 870 | 836 | 813 | 41.30 | 5.0% |
| Sample 3 | 3986 | 3724 | 3655 | 3712 | 3769 | 127.82 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For APCS (Source: Uniprot.Org, NCBI)
Gene Name
APCS
Full Name
Serum amyloid P-component
Weight
25.387kDa
Superfamily
pentraxin family
Alternative Names
9.5S alpha-1-glycoprotein; amyloid P component, serum; APCS; MGC88159; Pentraxin 2; PTX2; PTX2serum amyloid P-component; SAP; SAPpentaxin-related; Serum Amyloid P component APCS HEL-S-92n, PTX2, SAP amyloid P component, serum serum amyloid P-component|9.5S alpha-1-glycoprotein|epididymis secretory sperm binding protein Li 92n|pentaxin-related|pentraxin-2|pentraxin-related
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APCS, check out the APCS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APCS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SAP/PTX2/APCS ELISA Kit PicoKine® (EK1209)
Hello CJ!
EK1209 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats
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Customer Q&As
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6 Customer Q&As for Rat SAP/PTX2/APCS ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-11-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-11-02
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for Pentraxin 2 ELISA after freezing?
H. Anand
Verified customer
Asked: 2019-04-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Pentraxin 2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-04-05
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Pentraxin 2 in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
G. Johnson
Verified customer
Asked: 2018-04-24
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Pentraxin 2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-04-24
Question
Q: how to proceed with the analysis of ELISA data? I measured Pentraxin 2 level in serum.
Verified Customer
Verified customer
Asked: 2016-08-01
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-08-01
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Chakrabarti
Verified customer
Asked: 2014-08-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-08-01
