Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®

Uteroglobin/SCGB1A1 ELISA kit for Rat

Rat SCGB1A1/uteroglobin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1636
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®

View all Uteroglobin/SCGB1A1 ELISA kits

SKU/Catalog Number

EK1636

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat SCGB1A1/uteroglobin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1636)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S20-V96

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1636 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1636 is reactive to SCGB1A1 in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK1636 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Uteroglobin/SCGB1A1

SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.

Kit Components

Catalog Number Description Quantity
EK1636-CAP Anti-Rat SCGB1A1 Pre-coated 96-well strip microplate 1
EK1636-ST Rat SCGB1A1 Standard 2 vials, 10 ng/tube
EK1636-DA Rat SCGB1A1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0420.0750.0990.1540.2630.5221.1352.156

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Uteroglobin/SCGB1A1, Dilution ratio of 1:10, concentration in serum and plasma is around 20 ng/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat SCGB1A1/uteroglobin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2201610449024116354849
Standard deviation8.8123.97298.6%410.36140.61374.3%2
CV (%)4%7.7%6.6%4.3%8.6%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Uteroglobin/SCGB1A1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122025825925324716.036.4%
Sample 21610148313801552150685.635.6%
Sample 344904875448748494675186.973.9%
*number of samples for each test n=16.

Gene/Protein Information For SCGB1A1 (Source: Uniprot.Org, NCBI)

Gene Name

SCGB1A1

Full Name

Uteroglobin

Weight

9.994kDa

Superfamily

secretoglobin family

Alternative Names

Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin SCGB1A1 CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1 secretoglobin family 1A member 1 uteroglobin|blastokinin|clara cell phospholipid-binding protein|clara cells 10 kDa secretory protein|secretoglobin, family 1A, member 1 (uteroglobin)|urinary protein 1|urine protein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SCGB1A1, check out the SCGB1A1 Infographic

SCGB1A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SCGB1A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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6 Customer Q&As for Rat SCGB1A1/uteroglobin ELISA Kit PicoKine®

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-12-12

Question

Q: how to analyze ELISA data? I have performed Uteroglobin level in serum.

C. Kulkarni

Verified customer

Asked: 2019-09-16

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-16

Question

Q: if the enzyme conjugated Uteroglobin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Uteroglobin antigen?

R. Campbell

Verified customer

Asked: 2018-04-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-19

Question

Q: how can I thaw whole blood sample for Uteroglobin ELISA after freezing?

D. Garcia

Verified customer

Asked: 2016-10-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Uteroglobin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-10-20

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

D. Jackson

Verified customer

Asked: 2016-10-15

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-10-15

Question

Q: can I use citrate plasma as samples in Rat Uteroglobin Picokine® ELISA Kit (Catalog # EK1636)?

Verified Customer

Verified customer

Asked: 2016-09-14

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Uteroglobin causing disruption of its protein structure. Uteroglobin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Uteroglobin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-09-14

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