Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

Rat SEMA3F ELISA Kit PicoKine™ (96 Tests). Quantitate Rat SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1735
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

Product Name

Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

See all Semaphorin 3F products

SKU/Catalog Number

EK1735

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat SEMA3F ELISA Kit PicoKine™ (96 Tests). Quantitate Rat SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1735)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A19-T785

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1735 is reactive to Sema3f in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA or citrate)

Application Guarantee

EK1735 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1735-CAP Anti-Rat Sema3f Pre-coated 96-well strip microplate 1
EK1735-ST Rat Sema3f Standard 2 vials, 10 ng/tube
EK1735-DA Rat Sema3f Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.0790.1170.2140.4030.7011.2182.037

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Semaphorin 3F, Dilution ratio of 1:1, concentration in serum is around 200pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5822791959236947
Standard deviation3.0212.0343.193.6017.4648.30
CV (%)5.2%5.3%4.7%6.1%7.4%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 3F in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158545554551.632.9%
Sample 22272232172252233.741.6%
Sample 391993796497294821.172.2%
*number of samples for each test n=16.

Gene/Protein Information For Sema3f (Source: Uniprot.Org, NCBI)

Uniprot ID

Q13275

Gene ID

6405

Gene Name

Sema3f

Full Name

Semaphorin-3F

Weight

88.381kDa

Superfamily

semaphorin family

Alternative Names

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema III/F; sema IV; Sema3F; SEMA4; SEMA-IV; SEMAK; Semaphorin 3F; semaphorin III/F; semaphorin IV SEMA3F SEMA-IV, SEMA4, SEMAK semaphorin 3F semaphorin-3F|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F|semaphorin III/F|semaphorin IV

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Sema3f, check out the Sema3f Infographic

Sema3f infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Sema3f: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1735

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

8 Customer Q&As for Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-10-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-10-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Wilson

Verified customer

Asked: 2020-08-27

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-27

Question

Q: how to thaw whole blood sample for Semaphorin 3F ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-10-24

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Semaphorin 3F for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-10-24

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Semaphorin 3F using the EK1735 Rat Semaphorin 3F Picokine® ELISA Kit?

M. Zhou

Verified customer

Asked: 2019-10-18

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2019-10-18

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-05-08

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-08

Question

Q: if the enzyme conjugated Semaphorin 3F antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Semaphorin 3F antigen?

Verified Customer

Verified customer

Asked: 2018-09-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-09-10

Question

Q: how to analyze ELISA data? I measured Semaphorin 3F level in plasma.

D. Harris

Verified customer

Asked: 2018-07-20

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-07-20

Question

Q: Can Semaphorin 3F ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

N. Mangal

Verified customer

Asked: 2015-08-16

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2015-08-16

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1735
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.