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Product Info Summary
| SKU: | EK1735 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA or citrate) |
Product info
Product Name
Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine®
View all Semaphorin 3F ELISA kits
SKU/Catalog Number
EK1735
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SEMA3F ELISA Kit PicoKine™ (96 Tests). Quantitate Rat SEMA3F in cell culture supernatants, serum and plasma (heparin, EDTA or citrate) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1735)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A19-T785
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1735 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1735 is reactive to SEMA3F in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA or citrate)
Application Guarantee
EK1735 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Semaphorin 3F
Semaphorin-3F is a protein that in humans is encoded by the SEMA3F gene. It is mapped to 8q32. The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues. Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin 3 family, consisting of human semaphorins 3A-G, chicken collapsin, and mouse semaphorins 3A-G, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones through an interaction with a collapsin response mediator protein of relative molecular mass 62K , a putative homolog of an axonal guidance associated UNC33 gene product. SEMA3F is a secreted member of the semaphorin III family.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1735-CAP | Anti-Rat SEMA3F Pre-coated 96-well strip microplate | 1 |
| EK1735-ST | Rat SEMA3F Standard | 2 vials, 10 ng/tube |
| EK1735-DA | Rat SEMA3F Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.079 | 0.117 | 0.214 | 0.403 | 0.701 | 1.218 | 2.037 |
Data Example Images
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Rat SEMA3F PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Semaphorin 3F, Dilution ratio of 1:1, concentration in serum and plasma is around 200 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 227 | 919 | 59 | 236 | 947 |
| Standard deviation | 3.02 | 12.03 | 43.19 | 3.60 | 17.46 | 48.30 |
| CV (%) | 5.2% | 5.3% | 4.7% | 6.1% | 7.4% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 3F in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 54 | 55 | 54 | 55 | 1.63 | 2.9% |
| Sample 2 | 227 | 223 | 217 | 225 | 223 | 3.74 | 1.6% |
| Sample 3 | 919 | 937 | 964 | 972 | 948 | 21.17 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For SEMA3F (Source: Uniprot.Org, NCBI)
Gene Name
SEMA3F
Full Name
Semaphorin-3F
Weight
88.381kDa
Superfamily
semaphorin family
Alternative Names
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema III/F; sema IV; Sema3F; SEMA4; SEMA-IV; SEMAK; Semaphorin 3F; semaphorin III/F; semaphorin IV SEMA3F SEMA-IV, SEMA4, SEMAK semaphorin 3F semaphorin-3F|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F|semaphorin III/F|semaphorin IV
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SEMA3F, check out the SEMA3F Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SEMA3F: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine® (EK1735)
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No publications found for EK1735
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Rat SEMA3F/Semaphorin-3F ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-10-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-10-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Wilson
Verified customer
Asked: 2020-08-27
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-27
Question
Q: how to thaw whole blood sample for Semaphorin 3F ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Semaphorin 3F for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-10-24
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Semaphorin 3F using the EK1735 Rat Semaphorin 3F Picokine® ELISA Kit?
M. Zhou
Verified customer
Asked: 2019-10-18
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-10-18
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-08
Question
Q: if the enzyme conjugated Semaphorin 3F antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Semaphorin 3F antigen?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-10
Question
Q: how to analyze ELISA data? I measured Semaphorin 3F level in plasma.
D. Harris
Verified customer
Asked: 2018-07-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-07-20
Question
Q: Can Semaphorin 3F ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
N. Mangal
Verified customer
Asked: 2015-08-16
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2015-08-16
