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Product Info Summary
| SKU: | EK2059 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Rat SEMA6C ELISA Kit PicoKine®
View all Semaphorin 6C ELISA kits
SKU/Catalog Number
EK2059
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SEMA6C ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Sema6c in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SEMA6C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2059)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A24-P635
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2059 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2059 is reactive to SEMA6C in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2059 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Semaphorin 6C
Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 2q34. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2059-CAP | Anti-Rat SEMA6C Pre-coated 96-well strip microplate | 1 |
| EK2059-ST | Rat SEMA6C Standard | 2 vials, 10 ng/tube |
| EK2059-DA | Rat SEMA6C Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.056 | 0.123 | 0.164 | 0.286 | 0.527 | 0.928 | 1.613 | 2.354 |
Data Example Images
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Rat SEMA6C PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SEMA6C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 116 | 431 | 1934 | 121 | 478 | 1957 |
| Standard deviation | 6.73 | 25.86 | 88.96 | 7.38 | 32.98 | 142.86 |
| CV (%) | 5.8% | 6.0% | 4.6% | 6.1% | 6.9% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 6C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 116 | 110 | 102 | 113 | 110 | 5.21 | 4.7% |
| Sample 2 | 431 | 426 | 431 | 378 | 416 | 22.32 | 5.3% |
| Sample 3 | 1934 | 1731 | 1763 | 1838 | 1816 | 78.16 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For SEMA6C (Source: Uniprot.Org, NCBI)
Gene Name
SEMA6C
Full Name
Semaphorin-6C
Weight
99.686kDa
Superfamily
semaphorin family
Alternative Names
KIAA1869; m-SemaY; m-SemaY2; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C; Sema Y; Sema6C; Semaphorin 6C; Semaphorin Y; semaphorin-6C; semaphorin-Y; SEMAY SEMA6C SEMAY, m-SemaY, m-SemaY2 semaphorin 6C semaphorin-6C|sema Y|sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C|semaphorin-Y
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SEMA6C, check out the SEMA6C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SEMA6C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SEMA6C ELISA Kit PicoKine® (EK2059)
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No publications found for EK2059
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Rat SEMA6C ELISA Kit PicoKine®
Question
Q: can I use EDTA plasma as samples in Rat Semaphorin 6C Picokine® ELISA Kit (Catalog # EK2059)?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Semaphorin 6C causing disruption of its protein structure. Semaphorin 6C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Semaphorin 6C ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-04
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-31
Question
Q: can you recommend the dilution ratio of serum samples for detection of Semaphorin 6C in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Semaphorin 6C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-09-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-09-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2015-09-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-09-09
