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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.|
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Rat SerpinE2 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat SerpinE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat SerpinE2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1886)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: S20-P397
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1886 is reactive to Serpine2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.
EK1886 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1886-CAP||Anti-Rat Serpine2 Pre-coated 96-well strip microplate||1|
|EK1886-ST||Rat Serpine2 Standard||2 vials, 50 ng/tube|
|EK1886-DA||Rat Serpine2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat SerpinE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin E2/PN1, Dilution ratio of 1:50, concentration in serum is 200-500ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SerpinE2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Serpin E2/PN1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Serpine2 (Source: Uniprot.Org, NCBI)
GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2 Serpine2|CRG, Gdnpn1, PI-7, Pn-1, Spin4|serpin family E member 2|glia-derived nexin|Clozapine Regulated Gene|GDN|Glia-derived nexin/protease nexin I|glia-derived nexin / preotease nexin I|peptidase inhibitor 7|plasminogen activator inhibitor type 1|protease nexin 1|protease nexin I|protease nexin PN-1|serine (or cysteine) peptidase inhibitor, clade E, member 2|serine (or cysteine) proteinase inhibitor, clade E, member 2|serine protease inhibitor 4|serpin E2|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2|serpin peptidase inhibitor, clade E, member 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Serpine2, check out the Serpine2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpine2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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