Rat SerpinE2 ELISA Kit PicoKine™

Rat SerpinE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml.

Product Info Summary

SKU: EK1886
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.

Product Name

Rat SerpinE2 ELISA Kit PicoKine™

See all Serpin E2/PN1 products

SKU/Catalog Number

EK1886

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat SerpinE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat SerpinE2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1886)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Sensitivity

<0.1 ng/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1886 is reactive to Serpine2 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.

Application Guarantee

EK1886 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1886-CAP Anti-Rat Serpine2 Pre-coated 96-well strip microplate 1
EK1886-ST Rat Serpine2 Standard 2 vials, 50 ng/tube
EK1886-DA Rat Serpine2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0890.1250.1810.2870.4490.8861.852.043

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Serpin E2/PN1, Dilution ratio of 1:50, concentration in serum is 200-500ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat SerpinE2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4132922916459530009451
Standard deviation17.35131.49650.6438.68165.00737.18
CV (%)4.2%4.5%7.1%6.5%5.5%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin E2/PN1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 141343841646343220.084.6%
Sample 22922291328273068293286.572.9%
Sample 391648948957088369129280.313.0%
*number of samples for each test n=16.

Gene/Protein Information For Serpine2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P07092

Gene ID

29366

Gene Name

Serpine2

Full Name

Glia-derived nexin

Weight

44.063kDa

Superfamily

serpin family

Alternative Names

GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2 Serpine2|CRG, Gdnpn1, PI-7, Pn-1, Spin4|serpin family E member 2|glia-derived nexin|Clozapine Regulated Gene|GDN|Glia-derived nexin/protease nexin I|glia-derived nexin / preotease nexin I|peptidase inhibitor 7|plasminogen activator inhibitor type 1|protease nexin 1|protease nexin I|protease nexin PN-1|serine (or cysteine) peptidase inhibitor, clade E, member 2|serine (or cysteine) proteinase inhibitor, clade E, member 2|serine protease inhibitor 4|serpin E2|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2|serpin peptidase inhibitor, clade E, member 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Serpine2, check out the Serpine2 Infographic

Serpine2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Serpine2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1886

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Rat SerpinE2 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Rat SerpinE2 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

10 Customer Q&As for Rat SerpinE2 ELISA Kit PicoKine™

Question

Q: is it okay to use heparin plasma as samples in Rat Serpin E2 Picokine® ELISA Kit (Catalog # EK1886)?

Verified Customer

Verified customer

Asked: 2020-12-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Serpin E2 causing disruption of its protein structure. Serpin E2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Serpin E2 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-12-08

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Zhou

Verified customer

Asked: 2020-08-12

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-12

Question

Q: if the enzyme conjugated Serpin E2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin E2 antigen?

G. Zhou

Verified customer

Asked: 2020-07-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-07-17

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Serpin E2 in Rat plasma,urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-06-17

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin E2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-06-17

Question

Q: which procedure should I follow in order to thaw whole blood sample for Serpin E2 ELISA after freezing?

G. Kapoor

Verified customer

Asked: 2019-11-12

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin E2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-11-12

Question

Q: What is the optimal O.D. value for Serpin E2 ELISA kit? I performed your Serpin E2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin E2 even though the O.D. values are not very high?

T. Bhasin

Verified customer

Asked: 2019-09-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin E2 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of Serpin E2 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.

Boster Scientific Support

Answered: 2019-09-12

Question

Q: how to analyze ELISA data? I have obtained Serpin E2 level in plasma,urine.

Verified Customer

Verified customer

Asked: 2019-03-19

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-19

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-01-25

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-11-15

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-11-15

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin E2 using the EK1886 Rat Serpin E2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-02-14

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2016-02-14

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1886
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.