Rat TGF-Beta 2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF-beta 2. 96wells/kit, with removable strips. Cited in 2 publication(s).
Rat TGF-Beta 2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes. All VEGF-receptors have seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. VEGFR-2 has a lower affinity for VEGF than the Flt-1 receptor, but a higher signaling activity. Mitogenic activity in endothelial cells is mainly mediated by VEGFR-2 leading to their proliferation.
Differential splicing of the flt-1 gene leads to the formation of a secreted, soluble variant of VEGFR-1 (sVEGFR-1). No naturally occuring, secreted forms of VEGFR-2 have so far been reported. The binding of VEGF165 to VEGFR-2 is dependent on heparin.
|Product Name||Rat TGF-Beta 2 ELISA Kit PicoKine™
See all TGF-beta 2 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Rat Tgf-Beta 2/TGFB2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Tgf-Beta 2/TGFB2 tissue specificity: Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. .|
|Cite This Product||Rat TGF-Beta 2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0982)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: A303-S414|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Tgfb2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||81809|
|Species Of This Entry||Rat|
|Protein Name||Transforming growth factor beta-2 proprotein|
|Alternative Names||TGF-beta 2|BSC-1 cell growth inhibitor; cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; MGC116892; polyergin; TGFB2; TGFbeta 2; TGF-beta 2; TGF-beta2; TGF-beta-2; transforming growth factor beta-2; transforming growth factor, beta 2|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Nitration; Phosphorothioation; Demethylation; Biotinylation; Reduction; Deacetylation; Dephosphorylation|
|Gene Location||13q26, on Chromasome 13, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Tgfb2 (Source: Uniprot.org, NCBI)
|Protein Function||Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.|
|Tissue Specificity||Isoform TGF-beta2B: Expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine.|
|Related Diseases||Neoplasms; Malignant Neoplasms; Fibrosis; Glaucoma; Cataract; Tissue Adhesions; Malignant Paraganglionic Neoplasm; Carcinoma; Cell Invasion; Cicatrix; Malignant Neoplasm Of Breast; Glioma; Inflammation; Aqueous Humor Disorders; Neoplasm Metastasis; Mammary Neoplasms; Glaucoma, Open-angle; Cell Transformation, Neoplastic; Brain Neoplasms; Tumor Angiogenesis|
|Related Pathways||Cell Proliferation; Pathogenesis; Secretion; Cell Cycle; Wound Healing; Angiogenesis; Cell Migration; Localization; Cell Differentiation; Cell Growth; Cell Adhesion; Cell Death; Aging; Regeneration; Catagen; Immune Response; Epithelial Cell Proliferation; Hair Cycle; Fibroblast Proliferation; Methylation|
|Background||Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.|
|Scientific References||PMID: 10899565 by Koishi K., et al. The expression and structure of TGF-beta2 transcripts in rat muscles.|
PMID: 11014222 by Konrad L., et al. Transforming growth factor-beta2 mediates mesenchymal-epithelial interactions of testicular somatic cells.
Kit Components And Assay QC Data Details Of Rat TGF-Beta 2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0982-CAP||96-well plate precoated with anti-Rat TGF-beta 2 antibody||1|
|EK0982-ST||lyophilized recombinant Rat TGF-beta 2 standard||10ng/tube|
|EK0982-DA||biotinylated anti-Rat TGF-beta 2 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0982 Rat TGF-Beta 2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat TGF-Beta 2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat TGF-Beta 2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Li X, Liu L, Shen Y, Wang T, Chen L, Xu D, Wen F. Biochem Biophys Res Commun. 2014 Oct 27;454(4):512-517. Doi: 10.1016/J.Bbrc.2014.10.106. [Epub Ahead Of Print] Microrna-26A Modulates Transforming Growth Factor Beta-1-Induced Proliferation In Huma...||PubMed ID 25451270|
|Berberine hydrochloride prevents postsurgery intestinal adhesion and inflammation in rats||PubMed ID 24676878|
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