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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat VEGFR3/FLT4 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat VEGFR3/FLT4 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flt4 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat VEGFR3/FLT4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1347)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y25-D770
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1347 is reactive to Flt4 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK1347 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR3/Flt-4
Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
|EK1347-CAP||Anti-Rat Flt4 Pre-coated 96-well strip microplate||1|
|EK1347-ST||Rat Flt4 Standard||2 vials, 10 ng/tube|
|EK1347-DA||Rat Flt4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat VEGFR3/FLT4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VEGFR3/Flt-4, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat VEGFR3/FLT4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VEGFR3/Flt-4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Flt4 (Source: Uniprot.Org, NCBI)
Vascular endothelial growth factor receptor 3
protein kinase superfamily
EC 2.7.10; EC 184.108.40.206; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4 Flt4|Vegfr3|Fms related receptor tyrosine kinase 4|vascular endothelial growth factor receptor 3|FLT-4|FMS-like tyrosine kinase 4|VEGFR-3|fms-related tyrosine kinase 4|tyrosine-protein kinase receptor FLT4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Flt4, check out the Flt4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Flt4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1347 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li C, Fan J, Song X, Zhang B, Chen Y, Li C, Mi K, Ma H, Song Y, Tao X, Li G. Plos One. 2013 Sep 11;8(9):E75388. Doi: 10.1371/Journal.Pone.0075388. Ecollection 2013. Expression Of Angiopoietin-2 And Vascular Endothelial Growth Factor Receptor-3 Cor...
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