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Product Info Summary
|Description:||Transient overexpression lysate of retinol binding protein 2, cellular (RBP2)|
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RBP2 (NM_004164) Human Over-expression Lysate
Transient overexpression lysate of retinol binding protein 2, cellular (RBP2)
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Cite This Product
RBP2 (NM_004164) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005948)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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RBP2 Human Over-expression Lysates NM_004164.
Western blot validation of overexpression lysate (LS005948) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RBP2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for RBP2 (Source: Uniprot.org, NCBI)
Retinol-binding protein 2
Cellular retinol-binding protein II; cellular; CRABP-II; CRBP2CRBP-II; retinol binding protein 2, cellular RBP2 CRABP-II, CRBP2, CRBPII, RBPC2 retinol binding protein 2 retinol-binding protein 2|CRBP-II|cellular retinol-binding protein II|retinol binding protein 2, cellular*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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