Product Info Summary
| SKU: | PROTQ06599-1 |
|---|---|
| Size: | Starting from 10ug |
| Source: | E.coli |
| Application: | SDS-PAGE, Bioactivity |
Product info
Product Name
Bovine recombinant TNF-alpha protein
SKU/Catalog Number
PROTQ06599-1
Size
Starting from 10ug
Tag
Non-Tagged
Description
Bovine recombinant TNF-alpha protein expressed in E.coli with Non-Tagged. Sequence domain: 78-234aa. Application(s): SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Cite This Product
Bovine recombinant TNF-alpha protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ06599-1)
Form
Liquid
Formulation
Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Predicted MW
17.5 kDa (158aa) confirmed by MALDI-TOF
Amino Acid Sequence
Bovine, Q06599, 78-234aa; MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIALBioactivity
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15ng/ml.Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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