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Product Info Summary
| SKU: | PROTP27652 |
|---|---|
| Size: | Starting from 10ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
renilla reniformis Luciferase Recombinant Protein
SKU/Catalog Number
PROTP27652
Size
Starting from 10ug
Tag
His-Tag
Description
renilla reniformis Luciferase Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-311aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
renilla reniformis Luciferase Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP27652)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
0.5mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
38.5 kDa (335aa) Confirmed by MALDI-TOF
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Renilla reniformis, P27652, 1-311aa;Bioactivity
Specific activity is >1X10^9 light units/mg. One luciferase enzyme unit will produce one Relative Light Unit (RLU) at pH7.5 at 25°C.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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