REQU Colorimetric Cell-Based ELISA

DPF2 ELISA kit, cell-based

The REQU Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor REQU protein expression profile in cells. The kit can be used for measuring the relative amounts of REQU in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on REQU.

Product Info Summary

SKU: EKC1890
Size: 1 kit, containing 1 plates and all necessary reagents
Reactive Species: Human, Mouse
Application: ELISA

Product Name

REQU Colorimetric Cell-Based ELISA

See all DPF2 products

SKU/Catalog Number

EKC1890

Size

1 kit, containing 1 plates and all necessary reagents

*Question: How many samples can I assay/run in this kit?

Description

The REQU Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor REQU protein expression profile in cells. The kit can be used for measuring the relative amounts of REQU in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on REQU.

Storage & Handling

4°C/6 Months

Cite This Product

REQU Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1890)

Clonality of Antibodies

See Datasheet for details

Sensitivity

>5000 cells

Assay Range

> 5000 Cells

Reactive Species

EKC1890 is reactive to DPF2 in Human, Mouse samples

Application Guarantee

EKC1890 is guaranteed for ELISA in Human, Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-DPF2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For DPF2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q92785

Gene ID

5977

Gene Name

DPF2

Full Name

Zinc finger protein ubi-d4

Weight

44.155kDa

Superfamily

requiem/DPF family

Alternative Names

Apoptosis response zinc finger protein; D4, zinc and double PHD fingers family 2BAF45D; MGC10180; Protein requiem; REQBRG1-associated factor 45D; requiem, apoptosis response zinc finger; ubi-d4; UBID4requiem, apoptosis response zinc finger gene; zinc finger protein ubi-d4 DPF2 CSS7, REQ, UBID4, ubi-d4 double PHD fingers 2 zinc finger protein ubi-d4|BAF45D|BRG1-associated factor 45D|D4, zinc and double PHD fingers family 2|apoptosis response zinc finger protein|protein requiem|requiem, apoptosis response zinc finger

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DPF2, check out the DPF2 Infographic

DPF2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DPF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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