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Product Info Summary
| SKU: | LS006280 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of S100 calcium binding protein A9 (S100A9) |
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Product info
Product Name
S100A9 (NM_002965) Human Over-expression Lysate
SKU/Catalog Number
LS006280
Size
100 µg
Description
Transient overexpression lysate of S100 calcium binding protein A9 (S100A9)
Storage & Handling
Cite This Product
S100A9 (NM_002965) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006280)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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S100A9 Human Over-expression Lysates NM_002965.
Western blot validation of overexpression lysate (LS006280) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with S100A9 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for S100A9 (Source: Uniprot.org, NCBI)
Gene Name
S100A9
Full Name
Protein S100-A9
Weight
13.242kDa
Superfamily
S-100 family
Alternative Names
CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; Human S100A9 Capture; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9 S100A9 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14 S100 calcium binding protein A9 protein S100-A9|MRP-14|calgranulin B|calprotectin L1H subunit|leukocyte L1 complex heavy chain|migration inhibitory factor-related protein 14
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on S100A9, check out the S100A9 Infographic
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In this infographic, you will see the following information for S100A9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For S100A9 (NM_002965) Human Over-expression Lysate (LS006280)
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