Product Info Summary
SKU: | LS009294 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of sphingosine-1-phosphate receptor 2 (S1PR2) |
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Product info
Product Name
S1PR2 (NM_004230) Human Over-expression Lysate
See all S1P2/EDG-5/S1PR2 products
SKU/Catalog Number
LS009294
Size
100 µg
Description
Transient overexpression lysate of sphingosine-1-phosphate receptor 2 (S1PR2)
Storage & Handling
Cite This Product
S1PR2 (NM_004230) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009294)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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S1PR2 Human Over-expression Lysates NM_004230.
Western blot validation of overexpression lysate (LS009294) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with S1PR2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for S1PR2 (Source: Uniprot.org, NCBI)
Gene Name
S1PR2
Full Name
Sphingosine 1-phosphate receptor 2
Weight
38.867kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
AGR16; EDG5; EDG-5; endothelial differentiation G-protein coupled receptor 5; endothelial differentiation, sphingolipid G-protein-coupled receptor, 5; GPCR13; GPCR18; H218; LPB2; S1P receptor 2; S1P receptor EDG5; S1P receptor Edg-5; S1P2; S1PR2; sphingosine 1-phosphate receptor 2; sphingosine 1-phosphate receptor Edg-5; sphingosine-1-phosphate receptor 2 S1PR2 AGR16, DFNB68, EDG-5, EDG5, Gpcr13, H218, LPB2, S1P2 sphingosine-1-phosphate receptor 2 sphingosine 1-phosphate receptor 2|CTD-2369P2.2|S1P receptor 2|S1P receptor EDG5|S1P receptor Edg-5|deafness, autosomal recessive 68|endothelial differentiation G-protein coupled receptor 5|endothelial differentiation, sphingolipid G-protein-coupled receptor, 5|sphingosine 1-phosphate receptor Edg-5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on S1PR2, check out the S1PR2 Infographic
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In this infographic, you will see the following information for S1PR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For S1PR2 (NM_004230) Human Over-expression Lysate (LS009294)
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