Product Info Summary
| SKU: | A02904 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-S1PR2 Antibody
SKU/Catalog Number
A02904
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-S1PR2 Antibody (Catalog # A02904). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
S1PR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-S1PR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02904)
Host
Rabbit
Contents
S1PR2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2. The immunogen is located within amino acids 270 - 320 of S1PR2.
Cross-reactivity
S1PR2 antibody is human, mouse and rat reactive.
Reactive Species
A02904 is reactive to S1PR2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
38.9 kDa
Background of S1PR2
The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02904 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μg/ml.
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Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μg/ml.
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Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μg/ml.
Red: S1PR2 Antibody (A02904)
Blue: DAPI staining
Specific Publications For Anti-S1PR2 Antibody (A02904)
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