SDS-PAGE Protein Loading Buffer 2X (Reducing)

SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system. Cited in 6 publication(s).

Product Info Summary

SKU AR0131-20
Pack size 20mL
FormLiquid

Overview

Product Name SDS-PAGE Protein Loading Buffer 2X (Reducing)
SKU/Catalog Number AR0131-20
Form Liquid
Size 20mL
Contents 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromphenol blue and 0.1 M Tris HCl, pH 6.8
Description SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) of denatured proteins in the Laemmli SDS-PAGE system.
Equivalent Millipore Sigma (Product No. S3401); Bio-Rad (Product No. 1610737)
Cite This Product SDS-PAGE Protein Loading Buffer 2X (Reducing) (Boster Biological Technology, Pleasanton CA, USA, Catalog # AR0131-20)
Storage Upon receipt store at -20°C. It is stable at -20°C for one year. Product is shipped on ice.

Assay Principle

Using bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a ready-to-use 2X solution. It contains 4% SDS, 20% glycerol, 200mM DTT, 0.01% bromphenol blue and 0.1 M Tris HCl. It can be used for SDS-PAGE protein loading of conventional proteins. It is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. It is sufficient to prepare 12 mL protein samples. And the whole sample preparation process can be finished within 5 minutes.

Assay Protocol

1. Equilibrate SDS-PAGE Protein Loading Buffer 2X to room temperature or thaw Loading Buffer in a water bath no higher than 30°C.

2. Mix one volume of SDS-PAGE Protein Loading Buffer 2X with one volume of protein sample (i.e. add 1mL protein sample into 1 mL Loading Buffer).

3. Boil sample for 3-5 min.
Note: If semitransparent viscous substance remains after boiling, boil sample for another 5-10 min or add 1X SDS-PAGE Protein Loading Buffer which is diluted from 2X SDS-PAGE Protein Loading Buffer to the sample and then boil for another 3-5 min.

4. Allow sample to cool to room temperature.

5. Load sample into the wells of the SDS-PAGE gel and begin electrophoresis.

6. Stop electrophoresis when bromophenol blue dye front reaches to the bottom of the gel.

General Notes

1. SDS-PAGE Protein Loading Buffer 2X contains DTT which has a slightly irritating odor.

2. SDS-PAGE Protein Loading Buffer 2X should be completely dissolved before use.

3. Please wear the lab coat and disposable gloves and operate in a laboratory hood.

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AR0131-20 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Peng J,Qin C,Tian SY,Peng JQ.MiR-93 inhibits the vascular calcification of chronic renal failure by suppression of Wnt/β-catenin pathway.Int Urol Nephrol.2021 Jun 17.doi:10.1007/s11255-021-02907-6.Epub ahead of print.PMID:34138419.
Species: Rat
AR0131-20 usage in article: APP:WB, SAMPLE:AORTA TISSUE, DILUTION:NA

Wang,L.,Yuan,X.,Lian,L.,Guo,H.,Zhang,H.,& Zhang,M.(2021).Knockdown of lncRNA NORAD inhibits the proliferation, inflammation and fibrosis of human mesangial cells under high‑glucose conditions by regulating the miR‑485/NRF1 axis.Experimental and Therapeutic Medicine,22,874.https://doi.org/10.3892/etm.2021.10306
Species: Human
AR0131-20 usage in article: APP:WB, SAMPLE:HMCS, DILUTION:NA

Yin Zhao,Meng Ye,Jingqiu Huang et al.CD82 Protects Against Glaucomatous Axonal Transport Deficits via mTORC1& nbsp;Activation in Mice,14 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-404388/v1]
Species: Human,Mouse
AR0131-20 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:NA

Hu Z,He L,Wei J,Su Y,Wang W,Fan Z,Xu J,Zhang Y,Wang Y,Peng M,Zhao K,Zhang H,Liu C. tmbim4 protects against triclocarban-induced embryonic toxicity in zebrafish by regulating autophagy and apoptosis. Environ Pollut.2021 Mar 5;277:116873.doi:10.1016/j.envpol.2021.116873.Epub ahead of print.PMID:33714789.
Species: Zebrafish
AR0131-20 usage in article: APP:WB, SAMPLE:LARVAE, DILUTION:NA

Li T,Jiao JJ,Su Q,Hölscher C,Zhang J,Yan XD,Zhao HM,Cai HY,Qi JS.A GLP-1/GIP/Gcg receptor triagonist improves memory behavior, as well as synaptic transmission, neuronal excitability and Ca2+ homeostasis in 3xTg-AD mice.Neuropharmacology.2020 Jun 15;170:1
Species: Mouse
AR0131-20 usage in article: APP:WB, SAMPLE:HIPPOCAMPI, DILUTION:1:1

Su Q,Li T,He PF,Lu XC,Yu Q,Gao QC,Wang ZJ,Wu MN,Yang D,Qi JS.Trichostatin A ameliorates Alzheimer‘s disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice.Alzheimers Res Ther.2021 Jan 4;13(
Species: Mouse
AR0131-20 usage in article: APP:WB, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

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