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Product Info Summary
| SKU: | LS023678 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1 |
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Product info
Product Name
SGK3 (NM_013257) Human Over-expression Lysate
SKU/Catalog Number
LS023678
Size
100 µg
Description
Transient overexpression lysate of serum/glucocorticoid regulated kinase family, member 3 (SGK3), transcript variant 1
Storage & Handling
Cite This Product
SGK3 (NM_013257) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023678)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SGK3 Human Over-expression Lysates NM_001033578.
Western blot validation of overexpression lysate (LS023678) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SGK3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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SGK3 Human Over-expression Lysates NM_013257.
Western blot validation of overexpression lysate (LS023678) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SGK3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
SGK3 Human Over-expression Lysates NM_170709.
Western blot validation of overexpression lysate (LS023678) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SGK3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SGK3 (Source: Uniprot.org, NCBI)
Gene Name
SGK3
Full Name
Serine/threonine-protein kinase Sgk3
Weight
57.108kDa
Superfamily
protein kinase superfamily
Alternative Names
CISK; DKFZp781N0293; EC 2.7.11; EC 2.7.11.1; serine/threonine-protein kinase Sgk3; serum/glucocorticoid regulated kinase 3; serum/glucocorticoid regulated kinase family, member 3; serum/glucocorticoid regulated kinase-like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK2; SGKLcytokine-independent survival kinase SGK3 CISK, SGK2, SGKL serum/glucocorticoid regulated kinase family member 3 serine/threonine-protein kinase Sgk3|cytokine-independent survival kinase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SGK3, check out the SGK3 Infographic
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In this infographic, you will see the following information for SGK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SGK3 (NM_013257) Human Over-expression Lysate (LS023678)
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