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Product Info Summary
| SKU: | LS023408 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (SIRT5), transcript variant 2 |
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Product info
Product Name
SIRT5 (NM_031244) Human Over-expression Lysate
See all Sirtuin 5/SIRT5 products
SKU/Catalog Number
LS023408
Size
100 µg
Description
Transient overexpression lysate of sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (SIRT5), transcript variant 2
Storage & Handling
Cite This Product
SIRT5 (NM_031244) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS023408)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SIRT5 Human Over-expression Lysates NM_001193267.
Western blot validation of overexpression lysate (LS023408) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIRT5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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SIRT5 Human Over-expression Lysates NM_012241.
Western blot validation of overexpression lysate (LS023408) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIRT5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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SIRT5 Human Over-expression Lysates NM_031244.
Western blot validation of overexpression lysate (LS023408) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SIRT5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SIRT5 (Source: Uniprot.org, NCBI)
Gene Name
SIRT5
Full Name
NAD-dependent protein deacylase sirtuin-5, mitochondrial
Weight
33.881kDa
Superfamily
sirtuin family
Alternative Names
EC 3.5.1; EC 3.5.1.-; FLJ36950; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; SIR2L5; sir2-like 5; SIR2-like protein 5; SIRT5; sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5; Sirtuin 5; sirtuin type 5 SIRT5 SIR2L5 sirtuin 5 NAD-dependent protein deacylase sirtuin-5, mitochondrial|NAD-dependent deacetylase sirtuin-5|NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial|silent mating type information regulation 2, S.cerevisiae, homolog 5|sir2-like 5|sirtuin type 5
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SIRT5, check out the SIRT5 Infographic
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In this infographic, you will see the following information for SIRT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SIRT5 (NM_031244) Human Over-expression Lysate (LS023408)
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