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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 15 (oligopeptide transporter), member 1 (SLC15A1)|
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SLC15A1 (NM_005073) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 15 (oligopeptide transporter), member 1 (SLC15A1)
Storage & Handling
Cite This Product
SLC15A1 (NM_005073) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006564)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC15A1 Human Over-expression Lysates NM_005073.
Western blot validation of overexpression lysate (LS006564) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC15A1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC15A1 (Source: Uniprot.org, NCBI)
Solute carrier family 15 member 1
PTR2/POT transporter (TC 2.A.17) family
Caco-2 oligopeptide transporter; HPECT1macrophage oligopeptide transporter PEPT1; HPEPT1; Intestinal H(+)/peptide cotransporter; Oligopeptide transporter, small intestine isoform; PEPT1; PEPT1intestinal H+/peptide cotransporter; Peptide transporter 1; SLC15A1; solute carrier family 15 (oligopeptide transporter), member 1; solute carrier family 15 member 1 SLC15A1 HPECT1, HPEPT1, PEPT1 solute carrier family 15 member 1 solute carrier family 15 member 1|Caco-2 oligopeptide transporter|intestinal H+/peptide cotransporter|macrophage oligopeptide transporter PEPT1|peptide transporter 1|solute carrier family 15 (oligopeptide transporter), member 1*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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