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Product Info Summary
|Description:||Transient overexpression lysate of solute carrier family 1 (glutamate transporter), member 7 (SLC1A7)|
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SLC1A7 (NM_006671) Human Over-expression Lysate
Transient overexpression lysate of solute carrier family 1 (glutamate transporter), member 7 (SLC1A7)
Storage & Handling
Cite This Product
SLC1A7 (NM_006671) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006512)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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SLC1A7 Human Over-expression Lysates NM_006671.
Western blot validation of overexpression lysate (LS006512) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC1A7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC1A7 (Source: Uniprot.org, NCBI)
Excitatory amino acid transporter 5
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family
AAAT; EAAT5; excitatory amino acid transporter 5 (retinal glutamate transporter); excitatory amino acid transporter 5a long; retinal glutamate transporter; solute carrier family 1 (glutamate transporter), member 7 SLC1A7 AAAT, EAAT5 solute carrier family 1 member 7 excitatory amino acid transporter 5|excitatory amino acid transporter 5 (retinal glutamate transporter)|retinal glutamate transporter|solute carrier family 1 (glutamate transporter), member 7*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
For more info on SLC1A7, check out the SLC1A7 Infographic
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No publications found for LS006512
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