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Product Info Summary
| SKU: | LS064754 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of SET and MYND domain containing 3 (SMYD3), transcript variant 2 |
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Product info
Product Name
SMYD3 (NM_022743) Human Over-expression Lysate
SKU/Catalog Number
LS064754
Size
100 µg
Description
Transient overexpression lysate of SET and MYND domain containing 3 (SMYD3), transcript variant 2
Storage & Handling
Cite This Product
SMYD3 (NM_022743) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS064754)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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SMYD3 Human Over-expression Lysates NM_022743.
Western blot validation of overexpression lysate (LS064754) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SMYD3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for SMYD3 (Source: Uniprot.org, NCBI)
Gene Name
SMYD3
Full Name
Histone-lysine N-methyltransferase SMYD3
Weight
49.097kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
bA74P14.1 (novel protein); bA74P14.1; EC 2.1.1; EC 2.1.1.43; FLJ21080; KMT3E; MGC104324; MYND domain containing 1; SET and MYND domain containing 3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND1; ZNFN3A1 SMYD3 KMT3E, ZMYND1, ZNFN3A1, bA74P14.1 SET and MYND domain containing 3 histone-lysine N-methyltransferase SMYD3|SET and MYND domain-containing protein 3|zinc finger MYND domain-containing protein 1|zinc finger protein, subfamily 3A (MYND domain containing), 1|zinc finger, MYND domain containing 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on SMYD3, check out the SMYD3 Infographic
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In this infographic, you will see the following information for SMYD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For SMYD3 (NM_022743) Human Over-expression Lysate (LS064754)
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No publications found for LS064754
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