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Product Info Summary
| SKU: | M02080 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SMYD3 Monoclonal Antibody
SKU/Catalog Number
M02080
BM5457 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SMYD3 Monoclonal Antibody catalog # M02080. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMYD3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02080)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADHE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SMYD3 Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Reactive Species
M02080 is reactive to SMYD3 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
49.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02080 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of SMYD3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Specific Publications For Anti-SMYD3 Monoclonal Antibody (M02080)
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5 Customer Q&As for Anti-SMYD3 Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02080?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of SMYD3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Does anti-SMYD3 Monoclonal antibody M02080 work on feline IF with colon melanoma?
L. Walker
Verified customer
Asked: 2017-05-23
Answer
Our lab technicians have not tested anti-SMYD3 Monoclonal antibody M02080 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SMYD3 Monoclonal antibody M02080 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon melanoma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-23
Question
I see that the anti-SMYD3 Monoclonal antibody M02080 works with IF, what is the protocol used to produce the result images on the product page?
L. Thomas
Verified customer
Asked: 2016-07-28
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-07-28
Question
I was wanting to use your anti-SMYD3 Monoclonal antibody for IF for mouse colon melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse colon melanoma identification?
W. Zhang
Verified customer
Asked: 2014-07-23
Answer
It shows on the product datasheet, M02080 anti-SMYD3 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse colon melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-23
Question
We want to test anti-SMYD3 Monoclonal antibody M02080 on mouse colon melanoma for research purposes, then I may be interested in using anti-SMYD3 Monoclonal antibody M02080 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Walker
Verified customer
Asked: 2014-06-10
Answer
The products we sell, including anti-SMYD3 Monoclonal antibody M02080, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-10
