SMYD3 Antibodies

AND SMYD3 ELISA kits, SMYD3 Proteins

Many biological assays use antibodies to detect SMYD3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with SMYD3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop SMYD3 antibodies...

We validate the specificity of these antibodies to SMYD3 by testing them on tissues known to express SMYD3 positively and negatively. Browse below to find the SMYD3 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for SMYD3? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the SMYD3 IHC Guide

SMYD3 Infographic by Boster Bio

All SMYD3 Products

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Product

Figures

Specificity

Applications

Price

Anti-SMYD3 Antibody Picoband®

Western blot analysis of SMYD3 using anti-SMYD3 antibody (PB9893)

IHC analysis of SMYD3 using anti-SMYD3 antibody (PB9893)

Human

IHC, WB

$370
Cat # PB9893
100 μg/vial

SMYD3 (NM_022743) Human Over-expression Lysate

Human

$628
Cat # LS064754
20 µg, 100 µg

Anti-SMYD3 Monoclonal Antibody

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature

Western blot analysis of SMYD3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate

Human, Mouse, Rat

Flow Cytometry, IP, IF, IHC, ICC, WB

$370
Cat # M02080
100 μl

SMYD3 (NM_022743) Human Recombinant Protein

Coomassie blue staining of purified SMYD3 protein

$1249
Cat # PROTQ9H7B4
20 µg

SMYD3 Overview

Facts about Histone-lysine N-methyltransferase SMYD3.

SMYD3 3D structure. Source: alphafold

Histone-lysine N-methyltransferase SMYD3 (SMYD3)

Histone methyltransferase. Specifically methylates'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068).

Additionally methylates'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609).

Gene Information

Human
Gene Name:	SMYD3
Uniprot:	Q9H7B4
Entrez:	64754

Family

Belongs to:

class V-like SAM-binding methyltransferase superfamily

Alternative Names

bA74P14.1 (novel protein); bA74P14.1; EC 2.1.1; EC 2.1.1.43; FLJ21080; KMT3E; MGC104324; MYND domain containing 1; SET and MYND domain containing 3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; ZMYND1; ZNFN3A1

Molecular Weight

Mass (kDA):
49.097 kDA

Genomic Context


Human

Location:	1q44
Sequence:	1; NC_000001.11 (245749340..246507279, complement)

Tissue Specificity

Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

Subcellular Localizations

Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.

Diseases

Malignant Neoplasms
Neoplasms
Carcinoma
Liver Carcinoma
Carcinogenesis
Malignant Neoplasm Of Breast
Liver Neoplasms
Colorectal Cancer
Mammary Neoplasms
Cell Transformation, Neoplastic
Neoplasm Invasiveness
Hepatitis
Malignant Paraganglionic Neoplasm

Cell Invasion
Neoplasm Metastasis
Colorectal Neoplasms
Variable Number Tandem Repeat
Cholangiocarcinoma
Hepatitis B
Microcephaly

Interacting Proteins

MAP3K2

Pathways

Methylation
Cell Proliferation
Histone Methylation
Cell Growth
Cell Cycle
Rna Interference
Cell Migration
Dna Methylation
Gene Silencing
Regulation Of Gene Expression
Cell Cycle Arrest
Reverse Transcription
Translation

Induction Of Apoptosis
Localization
Demethylation
Histone H3 Acetylation
Heart Development
Cell Adhesion
Histone Modification

PTMs

Methylation
Demethylation

Acetylation
Phosphorylation

Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SMYD3

References

PMID: 15235609 by Hamamoto R., et al. SMYD3 encodes a histone methyltransferase involved in the proliferation of cancer cells.

PMID: 22419068 by Van Aller G.S., et al. Smyd3 regulates cancer cell phenotypes and catalyzes histone H4 lysine 5 methylation.