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Product Info Summary
| SKU: | PROTP18430-1 |
|---|---|
| Size: | 5ug,20ug |
| Origin Species: | Swine |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Swine recombinant IL-1 alpha (Interleukin-1 alpha) protein, AF
View all IL1A recombinant proteins
SKU/Catalog Number
PROTP18430-1
Size
5ug,20ug
Tag
His Tag (C-term)
Description
Interleukin 1-alpha (IL-1α), also named hematopoietin 1, is a 17.65 kDa cytokine with 159 amino acid residues. IL-1α is mainly expressed in macrophages, neutrophils, epithelial cells, and endothelial cells. When IL-1α binds to the IL-1 receptor, It mediates a wide variety of biological processes such as cell division, angiogenesis, production of interleukin-2, and cellular sodium ion homeostasis. In addition, IL-1α also plays a critical role in immune responses, such as fever and inflammation, and triggers tumor necrosis factor-alpha.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Swine recombinant IL-1 alpha (Interleukin-1 alpha) protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP18430-1)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>98% as determined by SDS-PAGE.
Molecular weight
The protein has a calculated MW of 19.02 kDa. The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to induce D10.G4.1 cells proliferation. The ED₅₀ for this effect is 50 pg/mL.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
MSATYSFQSNMKYNFMRVINHQCILNDARNQSIIRDPSGQYLMAAVLNNLDEAVKFDMAAYTSNDDSQLPVTLRISETRLFVSAQNEDEPVLLKELPETPKTIKDETSLLFFWEKHGNMDYFKSAAHPKLFIATRQEKLVHMAPGLPSVTDFQILENQS with polyhistidine tag at the C-terminus.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant swine IL-1 alpha
Specific Publications For Swine recombinant IL-1 alpha (Interleukin-1 alpha) protein, AF (PROTP18430-1)
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