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Product Info Summary
| SKU: | LS006908 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of TATA box binding protein (TBP), transcript variant 1 |
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Product info
Product Name
TATA binding protein (TBP) (NM_003194) Human Over-expression Lysate
See all TATA binding protein TBP products
SKU/Catalog Number
LS006908
Size
100 µg
Description
Transient overexpression lysate of TATA box binding protein (TBP), transcript variant 1
Storage & Handling
Cite This Product
TATA binding protein (TBP) (NM_003194) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006908)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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TATA binding protein (TBP) Human Over-expression Lysates NM_003194.
Western blot validation of overexpression lysate (LS006908) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with TBP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for TBP (Source: Uniprot.org, NCBI)
Gene Name
TBP
Full Name
TATA-box-binding protein
Weight
37.698kDa
Superfamily
TBP family
Alternative Names
GTF2D; GTF2D1; HDL4; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TF2D; TFIIDMGC117320; Transcription initiation factor TFIID TBP subunit TBP GTF2D, GTF2D1, HDL4, SCA17, TFIID TATA-box binding protein TATA-box-binding protein|TATA sequence-binding protein|TATA-box binding protein N-terminal domain|TATA-box factor|transcription initiation factor TFIID TBP subunit
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TBP, check out the TBP Infographic
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In this infographic, you will see the following information for TBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TATA binding protein (TBP) (NM_003194) Human Over-expression Lysate (LS006908)
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