Anti-TATA binding protein TBP Antibody
Rabbit IgG polyclonal antibody for TATA-box-binding protein(TBP) detection. Tested with WB in Human;Mouse;Rat.
Anti-TATA binding protein TBP Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Product Name||Anti-TATA binding protein TBP Antibody
See all TATA binding protein TBP primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for TATA BINDING PROTEIN TBP/TBP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. TATA BINDING PROTEIN TBP/TBP information: Molecular Weight: 37698 MW; Subcellular Localization: Nucleus; Tissue Specificity: Widely expressed, with levels highest in the testis and ovary.|
|Cite This Product||Anti-TATA binding protein TBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1534)|
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|Anti-TATA binding protein TBP Antibody may replace the following items: sc 26142|sc 26141|sc 33736|sc 74596|sc 74595|sc 26143.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human TBP(227-246aa EEQSRLAARKYARVVQKLGF), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For TBP (Source: Uniprot.org, NCBI)
|NCBI Gene Id||6908|
|Species Of This Entry||Human|
|Protein Name||TATA-box-binding protein|
|Alternative Names||TATA binding protein TBP|GTF2D; GTF2D1; HDL4; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TF2D; TFIIDMGC117320; Transcription initiation factor TFIID TBP subunit|
|Post Tranlational Modifications||Phosphorylation; Acetylation; Cleavage; Oxidation; Methylation; Reduction; Dephosphorylation; Nitration; Carboxylation; Bromination; Hydroxylation; Ubiquitination; Deacetylation; Sumoylation; Biotinylation; Farnesylation; Glycosylation; Demethylation; Sulphation|
|Gene Location||6q27, on Chromasome 6, gene sequence: NC_000006.12 (170554333..170572870)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TBP (Source: Uniprot.org, NCBI)
|Protein Function||General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.|
|Tissue Specificity||Widely expressed, with levels highest in the testis and ovary.|
|Interacting Proteins||Cdx1; GTF2A1; GTF2A2; GTF2B; POU3F2; SET; TAF1; TAF12; TAF1A; TAF1B; TAF1C; TP53|
|Research Areas||Transcription Factors and Regulators|
|Related Diseases||Neoplasms; Malignant Neoplasms; Ataxia; Ataxia, Spinocerebellar; Trinucleotide Repeat Expansion; Carcinoma; Neurodegenerative Disorders; Punctate Inner Choroidopathy; Hiv Infections; Atrophy; Retinoblastoma; Huntington Disease; Immunologic Deficiency Syndromes; Malignant Paraganglionic Neoplasm; Mammary Neoplasms; Tuberculosis; Leukemia; Cytomegalovirus Infections; Diabetes Mellitus|
|Related Pathways||Cell Cycle; Localization; Cell Growth; Transport; Chromatin Remodeling; Pathogenesis; Cell Proliferation; Regulation Of Gene Expression; Reverse Transcription; Methylation; Dna Replication; Cell Differentiation; Translation; Proteolysis; Viral Transcription; Mitosis; Histone Acetylation; Dna Repair; Spermatogenesis; Rrna Transcription|
|Background||TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.|
|Scientific References||PMID: 2374612 by Hoffmann A., et al. Highly conserved core domain and unique N-terminus with presumptive regulatory motifs in a human TATA factor (TFIID).|
PMID: 2363050 by Peterson M.G., et al. Functional domains and upstream activation properties of cloned human TATA binding protein.
Our Boster Quality Guarantee for Anti-TATA binding protein TBP Antibody covers its use in the following applications.Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: MCF-7 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: MM231 Cell Lysate
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