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Product Info Summary
| SKU: | M00302 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody
SKU/Catalog Number
M00302
BM4259 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody catalog # M00302. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00302)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DOA-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TBP
Reactive Species
M00302 is reactive to TBP in Human, Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
37.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00302 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of TBP expression in K562 cell lysate.
Specific Publications For Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody (M00302)
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4 Customer Q&As for Anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal Antibody
Question
We are currently using anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody M00302 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
The anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody (M00302) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-25
Question
We have seen staining in rat fibroblast. Any tips? Is anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody supposed to stain fibroblast positively?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
According to literature fibroblast does express TBP. According to Uniprot.org, TBP is expressed in forebrain, fibroblast, uterus, among other tissues. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-10
Question
We are interested in using your anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody for transcription elongation from rna polymerase i promoter studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-28
Answer
We appreciate your inquiry. This M00302 anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-28
Question
My colleagues were well pleased with the WB result of your anti-TBP/Tata Binding Protein Tbp Rabbit Monoclonal antibody. However we have seen positive staining in forebrain nucleus. using this antibody. Is that expected? Could you tell me where is TBP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
According to literature, forebrain does express TBP. Generally TBP expresses in nucleus. Regarding which tissues have TBP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 2194289
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-11-21
