TFF3 Trefoil Factor-3 Human Recombinant Protein

Product Name

TFF3 Trefoil Factor-3 Human Recombinant Protein

View all TFF3 recombinant proteins

SKU/Catalog Number

PROTQ07654

Size

5ug, 20ug, 1mg

Description

TFF-3 Human Recombinant produced in E. coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

TFF3 Trefoil Factor-3 Human Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTQ07654)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.

Purity

Greater than 97.0% as determined by RP-HPLC and SDS-PAGE analysis.

Predicted MW

10.181kDa

Reconstitution

It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10µg/ml, corresponding to a specific activity of greater than 100IU/mg.

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Reconstitution

It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For TFF3 (Source: Uniprot.org, NCBI)

Alternative Names

HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal) TFF3 ITF, P1B, TFI trefoil factor 3 trefoil factor 3|polypeptide P1.B|trefoil factor 3 (intestinal)

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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