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Product Info Summary
| SKU: | LS007150 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of topoisomerase (DNA) I (TOP1) |
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Product info
Product Name
Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate
See all Topoisomerase I products
SKU/Catalog Number
LS007150
Size
100 µg
Description
Transient overexpression lysate of topoisomerase (DNA) I (TOP1)
Storage & Handling
Cite This Product
Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007150)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information for TOP1 (Source: Uniprot.org, NCBI)
Gene Name
TOP1
Full Name
DNA topoisomerase 1
Weight
90.726kDa
Superfamily
type IB topoisomerase family
Alternative Names
DNA topoisomerase 1; DNA topoisomerase I; EC 5.99.1.2; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase TOP1 TOPI DNA topoisomerase I DNA topoisomerase 1|Scl-70 antigen|topoisomerase (DNA) I|type I DNA topoisomerase
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on TOP1, check out the TOP1 Infographic
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In this infographic, you will see the following information for TOP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Topoisomerase I (TOP1) (NM_003286) Human Over-expression Lysate (LS007150)
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