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Product Info Summary
| SKU: | A00434-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA topoisomerase 1 TOP1 Antibody
SKU/Catalog Number
A00434-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA topoisomerase 1 TOP1 Antibody catalog # A00434-2. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA topoisomerase 1 TOP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00434-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
Reactive Species
A00434-2 is reactive to TOP1 in Human, Mouse
Calculated molecular weight
90.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00434-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:50-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Western blot analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Jurkat Lane 3: MCF-7
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ICC staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Specific Publications For Anti-DNA topoisomerase 1 TOP1 Antibody (A00434-2)
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Customer Q&As
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1 Customer Q&As for Anti-DNA topoisomerase 1 TOP1 Antibody
Question
I want to know whether Anti-DNA Topoisomerase 1 is different from anti-TOP1 antibody.
Verified customer
Asked: 2022-08-05
Answer
Anti-DNA Topoisomerase 1 is the same as anti-TOP1 antibody. Thank you.
Boster Scientific Support
Answered: 2022-08-05
