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Product Info Summary
| SKU: | BA1093 |
|---|---|
| Size: | 0.5ml |
| Application: | Flow Cytometry, IF |
Product info
| Product Name | TRITC Conjugated Avidin |
| SKU/Catalog Number | BA1093 |
| Pack Size | 0.5 ml |
| Form | Concentrated, Liquid |
| Concentration | 1 mg/ml |
| Product Description | Avidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. |
| Contents | 0.5 mg of TRITC conjugated avidin, F/P=3/1; 0.01 M PBS; 5 mg/mL BSA, 50% glycerol. And the isoelectric point of avidin reduced from 10 to 6.5 through chemical modification. Thus, avidin has very low non-specific absorption to tissue and cell. The emission and filtration wavelength of TRITC are 550 nm and 620 nm respectively. |
| Labeling Method | Avidin is conjugated to TRITC by means of a method described by Hijmans,W.,et al. (Reference: Hijmans,W.,et al. Clin.Exp.Immunol.,4, 457 (1969)) |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Applications | IF, Flow Cytometry |
| Cite This Product | TRITC Conjugated Avidin (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1093) |
Product Images
Validation Images & Assay Conditions
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Beta Catenin was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Beta Catenin Antigen Affinity purified polyclonal antibody (Catalog # PA1212) at 1 μg/mL. TRITC Conjugated Avidin (Catalog # BA1093) was used for visualization at 20μg/mL.
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PCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti-PCK Antigen Affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL. TRITC Conjugated Avidin (Catalog # BA1093) was used for visualization at 20μg/mL.
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