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Product Info Summary
| SKU: | LS007337 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ubiquitin protein ligase E3A (UBE3A), transcript variant 2 |
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Product info
Product Name
UBE3A (NM_000462) Human Over-expression Lysate
SKU/Catalog Number
LS007337
Size
100 µg
Description
Transient overexpression lysate of ubiquitin protein ligase E3A (UBE3A), transcript variant 2
Storage & Handling
Cite This Product
UBE3A (NM_000462) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS007337)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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UBE3A Human Over-expression Lysates NM_000462.
Western blot validation of overexpression lysate (LS007337) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UBE3A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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UBE3A Human Over-expression Lysates NM_130839.
Western blot validation of overexpression lysate (LS007337) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with UBE3A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for UBE3A (Source: Uniprot.org, NCBI)
Gene Name
UBE3A
Full Name
Ubiquitin-protein ligase E3A
Weight
100.688kDa
Alternative Names
ANCR; ANCREPVE6AP; AS; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; E6AP; E6-AP; EC 6.3.2.-; EPVE6AP; FLJ26981; HPVE6A; human papilloma virus E6-associated protein; Human papillomavirus E6-associated protein; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY-REN-54; UBE3A; ubiquitin protein ligase E3A; ubiquitin-protein ligase E3A UBE3A ANCR, AS, E6-AP, EPVE6AP, HPVE6A ubiquitin protein ligase E3A ubiquitin-protein ligase E3A|CTCL tumor antigen se37-2|E6AP ubiquitin-protein ligase|HECT-type ubiquitin transferase E3A|human papilloma virus E6-associated protein|human papillomavirus E6-associated protein|oncogenic protein-associated protein E6-AP|renal carcinoma antigen NY-REN-54
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on UBE3A, check out the UBE3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for UBE3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For UBE3A (NM_000462) Human Over-expression Lysate (LS007337)
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