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Product Info Summary
| SKU: | M00582 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-UBE3A Monoclonal Antibody
SKU/Catalog Number
M00582
BM5372 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-UBE3A Monoclonal Antibody catalog # M00582. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-UBE3A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00582)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADBA-21
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human UBE3A E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B.
Reactive Species
M00582 is reactive to UBE3A in Human, Mouse, Rat
Observed Molecular Weight
100 kDa
Calculated molecular weight
100.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00582 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
Validation Images & Assay Conditions
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Western blot analysis of UBE3A expression in K562 cell lysate.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Specific Publications For Anti-UBE3A Monoclonal Antibody (M00582)
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1 Customer Q&As for Anti-UBE3A Monoclonal Antibody
Question
We are currently using anti-UBE3A Monoclonal antibody M00582 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
The anti-UBE3A Monoclonal antibody (M00582) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-23
