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Product Info Summary
| SKU: | BA1045 |
|---|---|
| Size: | 10mg |
| Host: | Rabbit |
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Product info
| Product Name | AffiniPure Rabbit IgG (H+L) |
| SKU/Catalog Number | BA1045 |
| Pack Size | 10 mg |
| Form | lyophilized |
| Purity | The antibody was purified from antiserum by Protein A. |
| Host | Rabbit |
| Clone | Polyclonal |
| Contents | 10 mg of affinity purified specific antibody (purification>99%). 0.01M PBS. |
| Usage Method | Recommended Dilutions: Optimal working dilutions must be determined by end users. Working solution can not be preserved for long after being diluted with 0.01M PBS buffer solution (pH7.0-7.6) or 1% sodium acetate. After adding preservatives (such as 0.05% NaN3) and reagent grade BSA to 1%, the antibody can be preserved for a long time. When labeling antibodies, such as HRP, FITC, Biotin, etc., the reagents should not contain BSA and NaN3. |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Applications | IHC, IF, ICC, WB, ELISA |
| Cite This Product | Rabbit IgG (H+L) (Boster Biological Technology, Pleasanton CA, USA, Catalog # BA1045) |
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Validation Images & Assay Conditions
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1 Customer Q&As for Rabbit IgG Isotype Control (H+L)
Question
In which range have you tested it? A customer is getting unspecified signals at a dilution of 1:700 (--> 1,4
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The suggested dilution ratio of BA1045 is 1:1000. The non-specific staining might be caused by the secondary antibody (2nd antibody: goat anti-rabbit IgG ) or the binding between Strep HRP and endogenous biotin in the sample. We suggest the customer run a negative control by using PBS to substitute the primary antibody to see if there is any non-specific staining.
Boster Scientific Support
Answered: 2019-11-08
