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Product Info Summary
|Description:||Transient overexpression lysate of zinc finger protein 354A (ZNF354A)|
ZNF354A (NM_005649) Human Over-expression Lysate
Transient overexpression lysate of zinc finger protein 354A (ZNF354A)
Storage & Handling
Cite This Product
ZNF354A (NM_005649) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006940)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ZNF354A Human Over-expression Lysates NM_005649.
Western blot validation of overexpression lysate (LS006940) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ZNF354A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ZNF354A (Source: Uniprot.org, NCBI)
Zinc finger protein 354A
krueppel C2H2-type zinc-finger protein family
G0/G1 switch regulatory protein 24; G0S24tristetraprolin; Growth factor-inducible nuclear protein NUP475; NUP475; Protein TIS11A; RNF162Atristetraproline; TIS11A; TIS11zfp-36; TTPGOS24; Zfp-36; Zinc finger protein 36 homolog; zinc finger protein 36, C3H type, homolog (mouse); zinc finger protein, C3H type, 36 homolog (mouse); zinc finger protein, C3H type, 36 homolog ZNF354A EZNF, HEL104, HKL1, KID-1, KID1, TCF17 zinc finger protein 354A zinc finger protein 354A|epididymis luminal protein 104|transcription factor 17|zinc finger protein eZNF*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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