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Product Info Summary
|Description:||Transient overexpression lysate of acrosin binding protein (ACRBP)|
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ACRBP (NM_032489) Human Over-expression Lysate
Transient overexpression lysate of acrosin binding protein (ACRBP)
Storage & Handling
Cite This Product
ACRBP (NM_032489) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084519)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ACRBP Human Over-expression Lysates NM_032489.
Western blot validation of overexpression lysate (LS084519) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ACRBP (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ACRBP (Source: Uniprot.org, NCBI)
acrosin binding protein; acrosin-binding protein; Cancer/testis antigen 23; Cancer/testis antigen OY-TES-1; CT23FLJ51160; OY-TES-1; proacrosin binding protein sp32; Proacrosin-binding protein sp32; SP32 Acrbp|OY-TES, OY-TES-1, sp, sp32|proacrosin binding protein|acrosin-binding protein|acrosin-binding protein, 60 kDa form*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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