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Product Info Summary
|Description:||Transient overexpression lysate of acyl-CoA synthetase bubblegum family member 2 (ACSBG2)|
ACSBG2 (NM_030924) Human Over-expression Lysate
Transient overexpression lysate of acyl-CoA synthetase bubblegum family member 2 (ACSBG2)
Storage & Handling
Cite This Product
ACSBG2 (NM_030924) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS081616)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ACSBG2 Human Over-expression Lysates NM_030924.
Western blot validation of overexpression lysate (LS081616) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ACSBG2 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ACSBG2 (Source: Uniprot.org, NCBI)
Long-chain-fatty-acid--CoA ligase ACSBG2
ATP-dependent AMP-binding enzyme family
acyl-CoA synthetase bubblegum family member 2BRGL; BGRMGC111089; Bubblegum-related protein; DKFZp434K1635; EC 188.8.131.52; long-chain-fatty-acid--CoA ligase ACSBG2; PRTD-NY3PRTDNY3 ACSBG2 BGR, BRGL, PRTD-NY3, PRTDNY3 acyl-CoA synthetase bubblegum family member 2 long-chain-fatty-acid--CoA ligase ACSBG2|arachidonate--CoA ligase ACSBG2|bubblegum-related protein|testicular tissue protein Li 8*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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