ACTL6A Colorimetric Cell-Based ELISA

BAF53A ELISA kit, cell-based

The ACTL6A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ACTL6A protein expression profile in cells. The kit can be used for measuring the relative amounts of ACTL6A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ACTL6A.

Product Info Summary

SKU: EKC1667
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

ACTL6A Colorimetric Cell-Based ELISA

See all BAF53A products

SKU/Catalog Number

EKC1667

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The ACTL6A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ACTL6A protein expression profile in cells. The kit can be used for measuring the relative amounts of ACTL6A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ACTL6A.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

ACTL6A Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1667)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1667 is reactive to ACTL6A in Human, Mouse, Rat samples

Application Guarantee

EKC1667 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-BAF53A Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For ACTL6A (Source: Uniprot.Org, NCBI)

Gene Name

ACTL6A

Full Name

Actin-like protein 6A

Weight

47.461kDa

Superfamily

actin family

Alternative Names

actin-like 6A; actin-like protein 6A; actin-related protein 4,53 kDa BRG1-associated factor A; Actin-related protein Baf53a; Actl6; Arp4; arpNbeta; BAF complex 53 kDa subunit; Baf53a; BAF53AACTL6; BAF53MGC5382; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80KARPN-BETA ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K actin like 6A actin-like protein 6A|53 kDa BRG1-associated factor A|BAF complex 53 kDa subunit|BAF53|BRG1-associated factor 53A|INO80 complex subunit K|actin-related protein 4|actin-related protein Baf53a|arpNbeta|hArpN beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACTL6A, check out the ACTL6A Infographic

ACTL6A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACTL6A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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