Product Info Summary
SKU: | EKC1667 |
---|---|
Size: | 1 kit, containing one 96-well plate and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
Customers Who Bought This Also Bought
Product info
Product Name
ACTL6A Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1667
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The ACTL6A Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ACTL6A protein expression profile in cells. The kit can be used for measuring the relative amounts of ACTL6A in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ACTL6A.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
ACTL6A Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1667)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1667 is reactive to ACTL6A in Human, Mouse, Rat samples
Application Guarantee
EKC1667 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-BAF53A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 6ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from Jurkat/COLO205 cells
Protein Target Info & Infographic
Gene/Protein Information For ACTL6A (Source: Uniprot.Org, NCBI)
Gene Name
ACTL6A
Full Name
Actin-like protein 6A
Weight
47.461kDa
Superfamily
actin family
Alternative Names
actin-like 6A; actin-like protein 6A; actin-related protein 4,53 kDa BRG1-associated factor A; Actin-related protein Baf53a; Actl6; Arp4; arpNbeta; BAF complex 53 kDa subunit; Baf53a; BAF53AACTL6; BAF53MGC5382; BRG1-associated factor 53A; hArpN beta; INO80 complex subunit K; INO80KARPN-BETA ACTL6A ACTL6, ARPN-BETA, Arp4, BAF53A, INO80K actin like 6A actin-like protein 6A|53 kDa BRG1-associated factor A|BAF complex 53 kDa subunit|BAF53|BRG1-associated factor 53A|INO80 complex subunit K|actin-related protein 4|actin-related protein Baf53a|arpNbeta|hArpN beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ACTL6A, check out the ACTL6A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACTL6A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ACTL6A Colorimetric Cell-Based ELISA (EKC1667)
Hello CJ!
No publications found for EKC1667
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used ACTL6A Colorimetric Cell-Based ELISA?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review ACTL6A Colorimetric Cell-Based ELISA
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question