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Product Info Summary
| SKU: | LS000135 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of adenosine A2a receptor (ADORA2A) |
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Product info
Product Name
Adenosine Receptor A2a (ADORA2A) (NM_000675) Human Over-expression Lysate
See all Adenosine A2aR products
SKU/Catalog Number
LS000135
Size
100 µg
Description
Transient overexpression lysate of adenosine A2a receptor (ADORA2A)
Storage & Handling
Cite This Product
Adenosine Receptor A2a (ADORA2A) (NM_000675) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000135)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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Adenosine Receptor A2a (ADORA2A) Human Over-expression Lysates NM_000675.
Western blot validation of overexpression lysate (LS000135) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADORA2A (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ADORA2A (Source: Uniprot.org, NCBI)
Gene Name
ADORA2A
Full Name
Adenosine receptor A2a
Weight
44.707kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
A2aR; adenosine A2 receptor; Adenosine A2a R; adenosine A2a receptor; Adenosine A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA 2; ADORA2; ADORA2A; hA2aR; RDC8 ADORA2A A2aR, ADORA2, RDC8 adenosine A2a receptor adenosine receptor A2a|adenosine receptor subtype A2a
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ADORA2A, check out the ADORA2A Infographic
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In this infographic, you will see the following information for ADORA2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Adenosine Receptor A2a (ADORA2A) (NM_000675) Human Over-expression Lysate (LS000135)
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No publications found for LS000135
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