Adrenergic Receptor alpha-2C Colorimetric Cell-Based ELISA

alpha-2C Adrenergic R/ADRA2C ELISA kit, cell-based

The Adrenergic Receptor alpha-2C Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Adrenergic Receptor alpha-2C protein expression profile in cells. The kit can be used for measuring the relative amounts of Adrenergic Receptor alpha-2C in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Adrenergic Receptor alpha-2C.

Product Info Summary

SKU: EKC1686
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

Adrenergic Receptor alpha-2C Colorimetric Cell-Based ELISA

See all alpha-2C Adrenergic R/ADRA2C products

SKU/Catalog Number

EKC1686

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Adrenergic Receptor alpha-2C Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Adrenergic Receptor alpha-2C protein expression profile in cells. The kit can be used for measuring the relative amounts of Adrenergic Receptor alpha-2C in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Adrenergic Receptor alpha-2C.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Adrenergic Receptor alpha-2C Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1686)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1686 is reactive to ADRA2C in Human, Mouse, Rat samples

Application Guarantee

EKC1686 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-alpha-2C Adrenergic R/ADRA2C Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For ADRA2C (Source: Uniprot.Org, NCBI)

Gene Name

ADRA2C

Full Name

Alpha-2C adrenergic receptor

Weight

49.522kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

a-2C AdrenergicR; ADRA2C; ADRA2L2; ADRA2L2alpha-2CAR; ADRA2RL2; ADRARL2; adrenergic, alpha-2C-, receptor; Alpha-2 adrenergic receptor subtype C4; alpha2-AR-C4; alpha-2C Adrenergic R; alpha-2C adrenergic receptor; alpha2C AdrenergicR; Alpha-2C adrenoceptor; Alpha-2C adrenoreceptor; alpha-2C-adrenergic receptor; ALPHA2CAR; Alpha-2CAR ADRA2C ADRA2L2, ADRA2RL2, ADRARL2, ALPHA2CAR adrenoceptor alpha 2C alpha-2C adrenergic receptor|adrenergic, alpha-2C-, receptor|alpha-2 adrenergic receptor subtype C4|alpha-2C adrenoceptor|alpha-2C adrenoreceptor|alpha-2CAR|alpha2-AR-C4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ADRA2C, check out the ADRA2C Infographic

ADRA2C infographic

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In this infographic you will see the following information for ADRA2C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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