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Product Info Summary
| SKU: | LS011047 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of adhesion regulating molecule 1 (ADRM1), transcript variant 1 |
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Product info
Product Name
ADRM1 (NM_007002) Human Over-expression Lysate
SKU/Catalog Number
LS011047
Size
100 µg
Description
Transient overexpression lysate of adhesion regulating molecule 1 (ADRM1), transcript variant 1
Storage & Handling
Cite This Product
ADRM1 (NM_007002) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS011047)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ADRM1 Human Over-expression Lysates NM_007002.
Western blot validation of overexpression lysate (LS011047) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADRM1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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ADRM1 Human Over-expression Lysates NM_175573.
Western blot validation of overexpression lysate (LS011047) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ADRM1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ADRM1 (Source: Uniprot.org, NCBI)
Gene Name
ADRM1
Full Name
Proteasomal ubiquitin receptor ADRM1
Weight
42.153kDa
Superfamily
ADRM1 family
Alternative Names
000 surface antigen; 110 kDa cell membrane glycoprotein; adhesion regulating molecule 1; Adhesion-regulating molecule 1; ARM1; ARM-1; Gp110; GP110hRpn13; M(r) 110; MGC29536; proteasomal ubiquitin receptor ADRM1; Proteasome regulatory particle non-ATPase 13; regulatory particle non-ATPase 13; Rpn13 homolog; Rpn13 ADRM1 ARM-1, ARM1, GP110, PSMD16 ADRM1 26S proteasome ubiquitin receptor proteasomal ubiquitin receptor ADRM1|110 kDa cell membrane glycoprotein|M(r) 110,000 surface antigen|adhesion regulating molecule 1|proteasome regulatory particle non-ATPase 13|proteasome ubiquitin receptor|rpn13 homolog
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ADRM1, check out the ADRM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADRM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ADRM1 (NM_007002) Human Over-expression Lysate (LS011047)
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