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Product Info Summary
| SKU: | LS155465 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of anterior gradient homolog 3 (Xenopus laevis) (AGR3) |
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Product info
Product Name
AGR3 (NM_176813) Human Over-expression Lysate
SKU/Catalog Number
LS155465
Size
100 µg
Description
Transient overexpression lysate of anterior gradient homolog 3 (Xenopus laevis) (AGR3)
Storage & Handling
Cite This Product
AGR3 (NM_176813) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS155465)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AGR3 Human Over-expression Lysates NM_176813.
Western blot validation of overexpression lysate (LS155465) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AGR3 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AGR3 (Source: Uniprot.org, NCBI)
Gene Name
AGR3
Full Name
Anterior gradient protein 3
Weight
19.171kDa
Superfamily
AGR family
Alternative Names
AG3; AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18 AGR3 AG-3, AG3, BCMP11, HAG3, PDIA18, hAG-3 anterior gradient 3, protein disulphide isomerase family member anterior gradient protein 3|anterior gradient homolog 3|anterior gradient protein 3 homolog|breast cancer membrane protein 11|protein disulfide isomerase family A, member 18
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AGR3, check out the AGR3 Infographic
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In this infographic, you will see the following information for AGR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AGR3 (NM_176813) Human Over-expression Lysate (LS155465)
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