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Product Info Summary
|Description:||Transient overexpression lysate of alkB, alkylation repair homolog 7 (E. coli) (ALKBH7)|
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ALKBH7 (NM_032306) Human Over-expression Lysate
Transient overexpression lysate of alkB, alkylation repair homolog 7 (E. coli) (ALKBH7)
Storage & Handling
Cite This Product
ALKBH7 (NM_032306) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS084266)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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ALKBH7 Human Over-expression Lysates NM_032306.
Western blot validation of overexpression lysate (LS084266) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ALKBH7 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for ALKBH7 (Source: Uniprot.org, NCBI)
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial
ABH7SPATA11; alkB, alkylation repair homolog 7 (E. coli); Alkylated DNA repair protein alkB homolog 7; EC 1.14.11.-; MGC10974; probable alpha-ketoglutarate-dependent dioxygenase ABH7; spermatogenesis associated 11; spermatogenesis cell proliferation related protein; Spermatogenesis cell proliferation-related protein; Spermatogenesis-associated protein 11; UNQ6002 ALKBH7 ABH7, SPATA11, UNQ6002 alkB homolog 7 alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial|alkB, alkylation repair homolog 7|alkylated DNA repair protein alkB homolog 7|probable alpha-ketoglutarate-dependent dioxygenase ABH7|spermatogenesis associated 11|spermatogenesis cell proliferation related protein|spermatogenesis-associated protein 11*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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