alpha 1 Spectrin (SPTA1) (NM_003126) Human Over-expression Lysate

Lysate of Spectrin alpha 1 over-expression cell lines, WB control

Transient overexpression lysate of spectrin, alpha, erythrocytic 1 (elliptocytosis 2) (SPTA1)

Product Info Summary

SKU: LS006708
Size: 100 µg
Expression Host: HEK293T
Tag:

C-Myc/DDK

Description: Transient overexpression lysate of spectrin, alpha, erythrocytic 1 (elliptocytosis 2) (SPTA1)

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Product Name

alpha 1 Spectrin (SPTA1) (NM_003126) Human Over-expression Lysate

See all Spectrin alpha 1 products

SKU/Catalog Number

LS006708

Size

100 µg

Description

Transient overexpression lysate of spectrin, alpha, erythrocytic 1 (elliptocytosis 2) (SPTA1)

Storage & Handling

Cite This Product

alpha 1 Spectrin (SPTA1) (NM_003126) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS006708)

Tag

C-Myc/DDK

Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)

Preparation

HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

Validation Images & Assay Conditions

Gene/Protein Information for SPTA1 (Source: Uniprot.org, NCBI)

Uniprot ID

P02549

Gene ID

6708

Gene Name

SPTA1

Full Name

Spectrin alpha chain, erythrocytic 1

Weight

280.014kDa

Superfamily

spectrin family

Alternative Names

alpha-I spectrin; EL2; erythrocyte; HS3; spectrin, alpha, erythrocytic 1 (elliptocytosis 2) SPTA1 EL2, HPP, HS3, SPH3, SPTA spectrin alpha, erythrocytic 1 spectrin alpha chain, erythrocytic 1|alpha-I spectrin|elliptocytosis 2|erythroid alpha-spectrin|spectrin alpha chain, erythrocyte

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on SPTA1, check out the SPTA1 Infographic

SPTA1 infographic

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In this infographic, you will see the following information for SPTA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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