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Product Info Summary
|Description:||Transient overexpression lysate of acyl-malonyl condensing enzyme 1-like 2 (AMAC1L2)|
AMAC1L2 (SLC35G5) (NM_054028) Human Over-expression Lysate
Transient overexpression lysate of acyl-malonyl condensing enzyme 1-like 2 (AMAC1L2)
Storage & Handling
Cite This Product
AMAC1L2 (SLC35G5) (NM_054028) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS083650)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Validation Images & Assay Conditions
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AMAC1L2 (SLC35G5) Human Over-expression Lysates NM_054028.
Western blot validation of overexpression lysate (LS083650) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with SLC35G5 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Gene/Protein Information for SLC35G5 (Source: Uniprot.org, NCBI)
Solute carrier family 35 member G5
SLC35G solute transporter family
Solute carrier family 35 member G5 SLC35G5 AMAC, AMAC1L2 solute carrier family 35 member G5 solute carrier family 35 member G5|acyl-malonyl condensing enzyme 1-like 2|acyl-malonyl-condensing enzyme 1-like protein 2|protein AMAC1L2*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".
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