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Product Info Summary
| SKU: | LS055626 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of autophagy/beclin-1 regulator 1 (AMBRA1) |
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Product info
Product Name
AMBRA1 (NM_017749) Human Over-expression Lysate
SKU/Catalog Number
LS055626
Size
100 µg
Description
Transient overexpression lysate of autophagy/beclin-1 regulator 1 (AMBRA1)
Storage & Handling
Cite This Product
AMBRA1 (NM_017749) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS055626)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AMBRA1 Human Over-expression Lysates NM_017749.
Western blot validation of overexpression lysate (LS055626) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AMBRA1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AMBRA1 (Source: Uniprot.org, NCBI)
Gene Name
AMBRA1
Full Name
Activating molecule in BECN1-regulated autophagy protein 1
Weight
142.507kDa
Alternative Names
activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736activating molecule in beclin-1-regulated autophagy; MGC33725; WD repeat domain 94; WDR94 AMBRA1 DCAF3, WDR94 autophagy and beclin 1 regulator 1 activating molecule in BECN1-regulated autophagy protein 1|DDB1 and CUL4 associated factor 3|WD repeat domain 94|activating molecule in beclin-1-regulated autophagy|autophagy/beclin-1 regulator 1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AMBRA1, check out the AMBRA1 Infographic
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In this infographic, you will see the following information for AMBRA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AMBRA1 (NM_017749) Human Over-expression Lysate (LS055626)
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No publications found for LS055626
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