Product Info Summary
SKU: | P02431 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody
SKU/Catalog Number
P02431
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (Catalog # P02431). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02431)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Clone Number
608
Isotype
IgG
Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02431 is reactive to YWHAB in Human, Mouse, Rat
Applications
P02431 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
28.082kDa
Background of YWHAB
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC: 1:100-1:500
Validation Images & Assay Conditions
Click image to see more details
Immunostaining of salamander retina showing labeling of 14-3-3 protein when phosphorylated at Ser58 in Muller glial cells in green and DNA in blue.
Click image to see more details
Western blot of rat brainstem lysate showing specific immunolabeling of the ~29 kDa 14-3-3 protein phosphorylated at Ser58 (-). The immunolabeling is blocked by the phosphopeptide used as the antigen (+) but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For YWHAB (Source: Uniprot.org, NCBI)
Gene Name
YWHAB
Full Name
14-3-3 protein beta/alpha
Weight
28.082kDa
Superfamily
14-3-3 family
Alternative Names
14-3-3 alpha; 14-3-3 beta; 14-3-3 protein beta/alpha; brain protein 14-3-3, beta isoform; GW128; HS1; KCIP-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide; YWHAA YWHAB GW128, HEL-S-1, HS1, KCIP-1, YWHAA tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta 14-3-3 protein beta/alpha|14-3-3 alpha|epididymis secretory protein Li 1|protein 1054|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YWHAB, check out the YWHAB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YWHAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (P02431)
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No publications found for P02431
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody
Question
We are currently using anti-Phospho-14-3-3 Protein (Ser58) antibody P02431 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?
J. Walker
Verified customer
Asked: 2013-01-09
Answer
The anti-Phospho-14-3-3 Protein (Ser58) antibody (P02431) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-09