Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody

YWHAB antibody

Boster Bio Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (Catalog # P02431). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P02431
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody

View all YWHAB Antibodies

SKU/Catalog Number

P02431

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (Catalog # P02431). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02431)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser58 of rat 14-3-3, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P02431 is reactive to YWHAB in Human, Mouse, Rat

Applications

P02431 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

28.082kDa

Background of YWHAB

14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, its dimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3 protein is regulated by site-specific serine phosphorylation (Woodcock et al., 2003)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:100-1:500

Validation Images & Assay Conditions

Gene/Protein Information For YWHAB (Source: Uniprot.org, NCBI)

Gene Name

YWHAB

Full Name

14-3-3 protein beta/alpha

Weight

28.082kDa

Superfamily

14-3-3 family

Alternative Names

14-3-3 alpha; 14-3-3 beta; 14-3-3 protein beta/alpha; brain protein 14-3-3, beta isoform; GW128; HS1; KCIP-1; Protein 1054; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alphapolypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, betapolypeptide; YWHAA YWHAB GW128, HEL-S-1, HS1, KCIP-1, YWHAA tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta 14-3-3 protein beta/alpha|14-3-3 alpha|epididymis secretory protein Li 1|protein 1054|protein kinase C inhibitor protein-1|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide|tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on YWHAB, check out the YWHAB Infographic

YWHAB infographic

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In this infographic, you will see the following information for YWHAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Phospho-14-3-3 Protein (Ser58) YWHAB Antibody

Question

We are currently using anti-Phospho-14-3-3 Protein (Ser58) antibody P02431 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?

J. Walker

Verified customer

Asked: 2013-01-09

Answer

The anti-Phospho-14-3-3 Protein (Ser58) antibody (P02431) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-01-09

Order DetailsPrice
P02431

100uL

$465

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